ArticleMolecular biology of the cell2009
Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- GPI-anchored Gas1 protein regulates cytosolic proteostasis in budding yeast.G3 (Bethesda, Md.) · 2024Article
- Fungal Cell Wall Proteins and Signaling Pathways Form a Cytoprotective Network to Combat Stresses.Journal of fungi (Basel, Switzerland) · 2021Review
- The Saccharomyces cerevisiae Ncw2 protein works on the chitin/β-glucan organisation of the cell wall.Antonie van Leeuwenhoek · 2021Article
- Determination of the lipid composition of the GPI anchor.PloS one · 2021Article
- Cdc1p is a Golgi-localized glycosylphosphatidylinositol-anchored protein remodelase.Molecular biology of the cell · 2020Article
- Ceramide chain length-dependent protein sorting into selective endoplasmic reticulum exit sites.Science advances · 2020Article
- The Post-Translational Modifications, Localization, and Mode of Attachment of Non-Covalently Bound Glucanosyltransglycosylases of Yeast Cell Wall as a Key to Understanding their Functioning.International journal of molecular sciences · 2020Article
- Simultaneous Nanoscale Imaging of Chemical and Architectural Heterogeneity on Yeast Cell Wall Particles.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Article
- FDA Approved Drug Library Screening Identifies Robenidine as a Repositionable Antifungal.Frontiers in microbiology · 2020Article
- The Glycosylphosphatidylinositol-AnchoredmSphere · 2019Article
- The natural anticancer agent cantharidin alters GPI-anchored protein sorting by targeting Cdc1-mediated remodeling in endoplasmic reticulum.The Journal of biological chemistry · 2019Article
- The PHR Family: The Role of Extracellular Transglycosylases in Shaping Candida albicans Cells.Journal of fungi (Basel, Switzerland) · 2017Review
- Role of Arf GTPases in fungal morphogenesis and virulence.PLoS pathogens · 2017Article
- The Cell Biology of Fission Yeast Septation.Microbiology and molecular biology reviews : MMBR · 2016Review
- Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling.BMC genomics · 2016Article
- Cell Wall Remodeling Enzymes Modulate Fungal Cell Wall Elasticity and Osmotic Stress Resistance.mBio · 2015Article
- Proteins involved in building, maintaining and remodeling of yeast cell walls.Current genetics · 2013Article
- Mitotic exit and separation of mother and daughter cells.Genetics · 2012Review
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gas1p is a glucan-elongase that plays a crucial role in yeast morphogenesis. It is predominantly anchored to the plasma membrane through a glycosylphosphatidylinositol, but a fraction was also found covalently bound to the cell wall. We have used fusions with the green fluorescent protein or red fluorescent protein (RFP) to determine its localization. Gas1p was present in microdomains of the plasma membrane, at the mother-bud neck and in the bud scars. By exploiting the instability of RFP-Gas1p, we identified mobile and immobile pools of Gas1p. Moreover, in chs3Delta cells the chitin ring and the cross-linked Gas1p were missing, but this unveiled an additional unexpected localization of Gas1p along the septum line in cells at cytokinesis. Localization of Gas1p was also perturbed in a chs2Delta mutant where a remedial septum is produced. Phenotypic analysis of cells expressing a fusion of Gas1p to a transmembrane domain unmasked new roles of the cell wall-bound Gas1p in the maintenance of the bud neck size and in cell separation. We present evidence that Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar. These results reveal a new mechanism of protein immobilization at specific sites of the cell envelope.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.