ArticleMolecular biology of the cell2009
The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- The Kelch Repeat Protein VdKeR1 Is Essential for Development, Ergosterol Metabolism, and Virulence inJournal of fungi (Basel, Switzerland) · 2024Article
- Cla4A, a Novel Regulator of Gene Expression Networks Required for Asexual and Insect-Pathogenic Lifecycles ofInternational journal of molecular sciences · 2024Article
- A Protein-Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20.International journal of molecular sciences · 2023Article
- FAS: assessing the similarity between proteins using multi-layered feature architectures.Bioinformatics (Oxford, England) · 2023Article
- Review
- A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress.Nucleic acids research · 2020Article
- Evolutionary dynamics in the fungal polarization network, a mechanistic perspective.Biophysical reviews · 2017Review
- From Lipid Homeostasis to Differentiation: Old and New Functions of the Zinc Cluster Proteins Ecm22, Upc2, Sut1 and Sut2.International journal of molecular sciences · 2017Review
- Sulfur Modifications of the Wobble UBiomolecules · 2017Review
- Active Interaction Mapping Reveals the Hierarchical Organization of Autophagy.Molecular cell · 2017Article
- The zinc cluster protein Sut1 contributes to filamentation in Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- The yeast acyltransferase Sct1p regulates fatty acid desaturation by competing with the desaturase Ole1p.Molecular biology of the cell · 2012Article
- Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Saccharomyces cerevisiae, the Rho-type GTPase Cdc42 regulates polarized growth through its effectors, including the p21-activated kinases (PAKs) Ste20, Cla4, and Skm1. Previously, we demonstrated that Ste20 interacts with several proteins involved in sterol synthesis that are crucial for cell polarization. Under anaerobic conditions, sterols cannot be synthesized and need to be imported into cells. Here, we show that Ste20, Cla4, and Skm1 form a complex with Sut1, a transcriptional regulator that promotes sterol uptake. All three PAKs can translocate into the nucleus and down-regulate the expression of genes involved in sterol uptake, including the Sut1 targets AUS1 and DAN1 by a novel mechanism. Consistently, deletion of either STE20, CLA4, or SKM1 results in an increased sterol influx and PAK overexpression inhibits sterol uptake. For Ste20, we demonstrate that the down-regulation of gene expression requires nuclear localization and kinase activity of Ste20. Furthermore, the Ste20-mediated control of expression of sterol uptake genes depends on SUT1 but is independent of a mitogen-activated protein kinase signaling cascade. Together, these observations suggest that PAKs translocate into the nucleus, where they modulate expression of sterol uptake genes via Sut1, thereby controlling sterol homeostasis.
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