ArticleMolecular biology of the cell2009
Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Rho-kinase and PKCα Inhibition Induces Primary Cilia Elongation and Alters the Behavior of Undifferentiated and Differentiated Temperature-sensitive Mouse Cochlear Cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Dynein activators and adaptors at a glance.Journal of cell science · 2019Review
- The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis.The Journal of cell biology · 2019Article
- Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons.The Journal of cell biology · 2019Article
- Loss of CENP-F Results in Dilated Cardiomyopathy with Severe Disruption of Cardiac Myocyte Architecture.Scientific reports · 2018Article
- Disentangling the molecular determinants for Cenp-F localization to nuclear pores and kinetochores.EMBO reports · 2018Article
- Regulation of Cenp-F localization to nuclear pores and kinetochores.Cell cycle (Georgetown, Tex.) · 2018Article
- CENP-F couples cargo to growing and shortening microtubule ends.Molecular biology of the cell · 2017Article
- Plk1-mediated stabilization of 53BP1 through USP7 regulates centrosome positioning to maintain bipolarity.Oncogene · 2017Article
- Hook2, a microtubule-binding protein, interacts with Par6α and controls centrosome orientation during polarized cell migration.Scientific reports · 2016Article
- Assembly and activation of dynein-dynactin by the cargo adaptor protein Hook3.The Journal of cell biology · 2016Article
- Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities.Molecular biology of the cell · 2016Article
- Centromere protein F includes two sites that couple efficiently to depolymerizing microtubules.The Journal of cell biology · 2015Article
- LRGUK-1 is required for basal body and manchette function during spermatogenesis and male fertility.PLoS genetics · 2015Article
- The kinetochore protein, CENPF, is mutated in human ciliopathy and microcephaly phenotypes.Journal of medical genetics · 2015Article
- Hook proteins: association with Alzheimer pathology and regulatory role of hook3 in amyloid beta generation.PloS one · 2015Article
- Gathering up meiotic telomeres: a novel function of the microtubule-organizing center.Cellular and molecular life sciences : CMLS · 2014Review
- The microtubule binding properties of CENP-E's C-terminus and CENP-F.Journal of molecular biology · 2013Article
- Golgi as an MTOC: making microtubules for its own good.Histochemistry and cell biology · 2013Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The microtubule (MT) network is essential in a broad spectrum of cellular functions. Many studies have linked CENP-F to MT-based activities as disruption of this protein leads to major changes in MT structure and function. Still, the basis of CENP-F regulation of the MT network remains elusive. Here, our studies reveal a novel and critical localization and role for CENP-F at the centrosome, the major MT organizing center (MTOC) of the cell. Using a yeast two-hybrid screen, we identify Hook2, a linker protein that is essential for regulation of the MT network at the centrosome, as a binding partner of CENP-F. With recently developed immunochemical reagents, we confirm this interaction and reveal the novel localization of CENP-F at the centrosome. Importantly, in this first report of CENP-F(-/-) cells, we demonstrate that ablation of CENP-F protein function eliminates MT repolymerization after standard nocodazole treatment. This inhibition of MT regrowth is centrosome specific because MT repolymerization is readily observed from the Golgi in CENP-F(-/-) cells. The centrosome-specific function of CENP-F in the regulation of MT growth is confirmed by expression of truncated CENP-F containing only the Hook2-binding domain. Furthermore, analysis of partially reconstituted MTOC asters in cells that escape complete repolymerization block shows that disruption of CENP-F function impacts MT nucleation and anchoring rather than promoting catastrophe. Our study reveals a major new localization and function of CENP-F at the centrosome that is likely to impact a broad array of MT-based actions in the cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.