ArticleThe journal of physical chemistry. B2009
Translational and rotational diffusion of a small globular protein under crowded conditions.
Article in The journal of physical chemistry. B, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 100 citations in OpenAlex.
- Rotational dynamics of a disk in a thin film of weakly nematic fluid subject to linear friction.The European physical journal. E, Soft matter · 2024Article
- Computational Approaches to Predict Protein-Protein Interactions in Crowded Cellular Environments.Chemical reviews · 2024Review
- Time-Resolved X-ray Observation of Intracellular Crystallized Protein in Living Animal.International journal of molecular sciences · 2023Article
- Location of dopamine in lipid bilayers and its relevance to neuromodulator function.Biophysical journal · 2023Article
- Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses.Nature communications · 2022Article
- Protein Fibrillation under Crowded Conditions.Biomolecules · 2022Review
- Biomolecular phase separation through the lens of sodium-23 NMR.Protein science : a publication of the Protein Society · 2021Article
- Fluorescence Anisotropy Decays and Microscale-Volume Viscometry Reveal the Compaction of Ribosome-Bound Nascent Proteins.The journal of physical chemistry. B · 2021Article
- Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy.Scientific reports · 2021Article
- Room temperature crystallization of amorphous polysiloxane using photodimerization.Chemical science · 2020Article
- Diffusive protein interactions in human versus bacterial cells.Current research in structural biology · 2020Article
- Liquid Metastable Precursors of Ibuprofen as Aqueous Nucleation Intermediates.Angewandte Chemie (International ed. in English) · 2019Article
- Article
- Whole-Cell Models and Simulations in Molecular Detail.Annual review of cell and developmental biology · 2019Review
- The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity.International journal of molecular sciences · 2019Review
- Effect of protein-protein interactions and solvent viscosity on the rotational diffusion of proteins in crowded environments.Physical chemistry chemical physics : PCCP · 2019Article
- Measuring translational diffusion ofEuropean biophysics journal : EBJ · 2018Article
- Macromolecular Crowding May Significantly Affect the Performance of an MRI Contrast Agent: AChemistryOpen · 2018Article
- Slow-Down in Diffusion in Crowded Protein Solutions Correlates with Transient Cluster Formation.The journal of physical chemistry. B · 2017Article
- Crowding in Cellular Environments at an Atomistic Level from Computer Simulations.The journal of physical chemistry. B · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Protein-protein interaction is the fundamental step of biological signal transduction. Interacting proteins find each other by diffusion. To gain insight into diffusion under the crowded conditions found in cells, we used nuclear magnetic resonance spectroscopy (NMR) to measure the effects of solvent additives on the translational and rotational diffusion of the 7.4 kDa globular protein, chymotrypsin inhibitor 2. The additives were glycerol and the macromolecular crowding agent, polyvinyl pyrrolidone (PVP). Both translational diffusion and rotational diffusion decrease with increasing solution viscosity. For glycerol, the decrease obeys the Stokes-Einstein and Stokes-Einstein Debye laws. Three types of deviation are observed for PVP: the decrease in diffusion with increased viscosity is less than predicted, this negative deviation is greater for rotational diffusion, and the negative deviation increases with increasing PVP molecular weight. We discuss our results in terms of other studies on the effects of macromolecules on globular protein diffusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.