ArticlePloS one2009
A microscope automated fluidic system to study bacterial processes in real time.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024Review
- Scaling of stochastic growth and division dynamics: A comparative study of individual rod-shaped cells in the Mother Machine and SChemostat platforms.Molecular biology of the cell · 2024Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- A divergent CheW confers plasticity to nucleoid-associated chemosensory arrays.PLoS genetics · 2019Article
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- Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software.Nature communications · 2018Article
- The nucleoid as a scaffold for the assembly of bacterial signaling complexes.PLoS genetics · 2017Article
- Fitness Trade-Offs in Competence Differentiation of Bacillus subtilis.Frontiers in microbiology · 2016Article
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- Single-Cell Analysis of Growth and Cell Division of the Anaerobe Desulfovibrio vulgaris Hildenborough.Frontiers in microbiology · 2015Article
- Functional organization of a multimodular bacterial chemosensory apparatus.PLoS genetics · 2014Article
- Characterization and resuscitation of 'non-culturable' cells of Legionella pneumophila.BMC microbiology · 2014Article
- Adaptive imaging cytometry to estimate parameters of gene networks models in systems and synthetic biology.PloS one · 2014Article
- A versatile class of cell surface directional motors gives rise to gliding motility and sporulation in Myxococcus xanthus.PLoS biology · 2013Article
- Direct live imaging of cell-cell protein transfer by transient outer membrane fusion in Myxococcus xanthus.eLife · 2013Article
- Single-cell analysis of cell viability after a biocide treatment unveils an absence of positive correlation between two commonly used viability markers.MicrobiologyOpen · 2013Article
- Microfabricated polyacrylamide devices for the controlled culture of growing cells and developing organisms.PloS one · 2013Article
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- Article
- Adaptation and preadaptation of Salmonella enterica to Bile.PLoS genetics · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most time lapse microscopy experiments studying bacterial processes ie growth, progression through the cell cycle and motility have been performed on thin nutrient agar pads. An important limitation of this approach is that dynamic perturbations of the experimental conditions cannot be easily performed. In eukaryotic cell biology, fluidic approaches have been largely used to study the impact of rapid environmental perturbations on live cells and in real time. However, all these approaches are not easily applicable to bacterial cells because the substrata are in all cases specific and also because microfluidics nanotechnology requires a complex lithography for the study of micrometer sized bacterial cells. In fact, in many cases agar is the experimental solid substratum on which bacteria can move or even grow. For these reasons, we designed a novel hybrid micro fluidic device that combines a thin agar pad and a custom flow chamber. By studying several examples, we show that this system allows real time analysis of a broad array of biological processes such as growth, development and motility. Thus, the flow chamber system will be an essential tool to study any process that take place on an agar surface at the single cell level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.