ArticleCellular and molecular neurobiology2010
Dominant-negative myosin Va impairs retrograde but not anterograde axonal transport of large dense core vesicles.
Article in Cellular and molecular neurobiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Axonal Transport Deficits in Parkinson's Disease: Insights from Neurotoxin, Genetic, and Sporadic Models.Brain sciences · 2026Review
- Roles of molecular motors in insulin-secreting beta cells.Current opinion in cell biology · 2025Review
- Neuroendocrine Control of Synaptic Transmission by PHAC-1 inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- A novel, rapidly progressive ataxia due to a spontaneous Myo5a mutation in mice impairs transport proteins and alters mitochondria.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Article
- Presynaptic perspective: Axonal transport defects in neurodevelopmental disorders.The Journal of cell biology · 2024Review
- Article
- Trafficking of hormones and trophic factors to secretory and extracellular vesicles: a historical perspective and new hypothesis.Extracellular vesicles and circulating nucleic acids · 2023Article
- Differential axonal trafficking of Neuropeptide Y-, LAMP1-, and RAB7-tagged organelles in vivo.eLife · 2022Article
- Nanoscale Organization of Vesicle Release at Central Synapses.Trends in neurosciences · 2019Review
- Secretory vesicle trafficking in awake and anaesthetized mice: differential speeds in axons versus synapses.The Journal of physiology · 2018Article
- Regulation of KIF1A-Driven Dense Core Vesicle Transport: CaCell reports · 2018Article
- Cargo crowding at actin-rich regions along axons causes local traffic jams.Traffic (Copenhagen, Denmark) · 2018Article
- The molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L.Scientific reports · 2017Article
- Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.PloS one · 2017Article
- Identification of Kinesin-1 Cargos Using Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2016Article
- The role of rab proteins in neuronal cells and in the trafficking of neurotrophin receptors.Membranes · 2014Review
- Structure meets function: actin filaments and myosin motors in the axon.Journal of neurochemistry · 2014Review
- Functions of class V myosins in neurons.The Journal of biological chemistry · 2013Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Axonal transport of peptide and hormone-containing large dense core vesicles (LDCVs) is known to be a microtubule-dependent process. Here, we suggest a role for the actin-based motor protein myosin Va specifically in retrograde axonal transport of LDCVs. Using live-cell imaging of transfected hippocampal neurons grown in culture, we measured the speed, transport direction, and the number of LDCVs that were labeled with ectopically expressed neuropeptide Y fused to EGFP. Upon expression of a dominant-negative tail construct of myosin Va, a general reduction of movement in both dendrites and axons was observed. In axons, it was particularly interesting that the retrograde speed of LDCVs was significantly impaired, although anterograde transport remained unchanged. Moreover, particles labeled with the dominant-negative construct often moved in the retrograde direction but rarely in the anterograde direction. We suggest a model where myosin Va acts as an actin-dependent vesicle motor that facilitates retrograde axonal transport.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.