ArticleExtremophiles : life under extreme conditions2010
Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus.
Article in Extremophiles : life under extreme conditions, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Article
- Origins of DNA replication.PLoS genetics · 2019Review
- Diversity of DNA Replication in the Archaea.Genes · 2017Review
- Genome Replication inFrontiers in microbiology · 2017Article
- Characterization of the replication initiator Orc1/Cdc6 from the Archaeon Picrophilus torridus.Journal of bacteriology · 2014Article
- DNA replication origins.Cold Spring Harbor perspectives in biology · 2013Review
- Mechanisms for initiating cellular DNA replication.Annual review of biochemistry · 2013Review
- Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex (Orc) on oriP of Epstein-Barr virus with purified proteins: stimulation by Cdc6 through its direct interaction with EBNA1.The Journal of biological chemistry · 2012Article
- Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.Applied and environmental microbiology · 2011Article
- Characterization of plasmid pPO1 from the hyperacidophile Picrophilus oshimae.Archaea (Vancouver, B.C.) · 2011Article
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Authors and funding
6 authors.
Funding
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Abstract
The initiation step is a key process to regulate the frequency of DNA replication. Although recent studies in Archaea defined the origin of DNA replication (oriC) and the Cdc6/Orc1 homolog as an origin recognition protein, the location and mechanism of duplex opening have remained unclear. We have found that Cdc6/Orc1 binds to oriC and unwinds duplex DNA in the hyperthermophilic archaeon Pyrococcus furiosus, by means of a P1 endonuclease assay. A primer extension analysis further revealed that this localized unwinding occurs in the oriC region at a specific site, which is 12-bp long and rich in adenine and thymine. This site is different from the predicted duplex unwinding element (DUE) that we reported previously. We also discovered that Cdc6/Orc1 induces topological changes in supercoiled oriC DNA, and that this process is dependent on the AAA+ domain. These results indicate that topological alterations of oriC DNA by Cdc6/Orc1 introduce a single-stranded region at the 12-mer site, that could possibly serve as an entry point for Mcm helicase.
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