ArticleJournal of biomolecular NMR2010
Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells.
Article in Journal of biomolecular NMR, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Glycoprotein Preparation by Heterologous Expression.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy.Plant biotechnology journal · 2022Article
- Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1.Journal of biomolecular NMR · 2022Article
- Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling.Biomolecules · 2020Article
- Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 inBiomolecules · 2020Article
- Structural characterization of a protein adsorbed on aluminum hydroxide adjuvant in vaccine formulation.NPJ vaccines · 2019Article
- Late metabolic precursors for selective aromatic residue labeling.Journal of biomolecular NMR · 2018Review
- Modern Technologies of Solution Nuclear Magnetic Resonance Spectroscopy for Three-dimensional Structure Determination of Proteins Open Avenues for Life Scientists.Computational and structural biotechnology journal · 2017Review
- Molecular Determinants of Tubulin's C-Terminal Tail Conformational Ensemble.ACS chemical biology · 2016Article
- DSF Guided Refolding As A Novel Method Of Protein Production.Scientific reports · 2016Article
- Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma.Molecular & cellular proteomics : MCP · 2015Article
- Affordable uniform isotope labeling with (2)H, (13)C and (15)N in insect cells.Journal of biomolecular NMR · 2015Article
- Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule.Journal of biomolecular NMR · 2015Article
- Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.Methods (San Diego, Calif.) · 2014Article
- Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra.Journal of biomolecular NMR · 2013Article
- Utilization of lysine ¹³C-methylation NMR for protein-protein interaction studies.Journal of biomolecular NMR · 2013Article
- Isotope labeling in mammalian cells.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Multi-dimensional NMR without coherence transfer: minimizing losses in large systems.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2011Article
- Mammalian production of an isotopically enriched outer domain of the HIV-1 gp120 glycoprotein for NMR spectroscopy.Journal of biomolecular NMR · 2011Article
- Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environment.Journal of biomolecular NMR · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The preparation of stable isotope-labeled proteins is necessary for the application of a wide variety of NMR methods, to study the structures and dynamics of proteins and protein complexes. The E. coli expression system is generally used for the production of isotope-labeled proteins, because of the advantages of ease of handling, rapid growth, high-level protein production, and low cost for isotope-labeling. However, many eukaryotic proteins are not functionally expressed in E. coli, due to problems related to disulfide bond formation, post-translational modifications, and folding. In such cases, other expression systems are required for producing proteins for biomolecular NMR analyses. In this paper, we review the recent advances in expression systems for isotopically labeled heterologous proteins, utilizing non-E. coli prokaryotic and eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.