ArticlePloS one2009
Lipid alterations in experimental murine colitis: role of ceramide and imipramine for matrix metalloproteinase-1 expression.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- CD39 and CD161 modulate Th17 responses in Crohn's disease.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- Fecal microbiota transplantation from Hezuo pigs alleviates intestinal inflammatory injury in Clostridium perfringens type C-infected piglets via modulation gut microbiota and intestinal barrier.BMC microbiology · 2026Article
- Unveiling the influence of lipidomes on inflammatory bowel disease: a bidirectional mendelian randomization study.BMC gastroenterology · 2025Article
- Mucosal and Plasma Metabolomes in New-onset Paediatric Inflammatory Bowel Disease: Correlations with Disease Characteristics and Plasma Inflammation Protein Markers.Journal of Crohn's & colitis · 2023Article
- Protective Effects of Protopanaxatriol Saponins on Ulcerative Colitis in Mouse Based on UPLC-Q/TOF-MS Serum and Colon Metabolomics.Molecules (Basel, Switzerland) · 2022Article
- Deoxysphingolipids Upregulate MMP-1, Downregulate TIMP-1, and Induce Cytotoxicity in Human Schwann Cells.Neuromolecular medicine · 2022Article
- Utilizing Sphingomyelinase Sensitizing Liposomes in Imaging Intestinal Inflammation in Dextran Sulfate Sodium-Induced Murine Colitis.Biomedicines · 2022Article
- Inhibition of acid sphingomyelinase by imipramine abolishes the synergy between metabolic syndrome and periodontitis on alveolar bone loss.Journal of periodontal research · 2022Article
- Fat of the Gut: Epithelial Phospholipids in Inflammatory Bowel Diseases.International journal of molecular sciences · 2021Review
- Protective effects of desipramine on alveolar bone in experimental periodontitis.Journal of periodontology · 2020Article
- G2A Signaling Dampens Colitic Inflammation via Production of IFN-γ.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- A lipidomics investigation into the intervention of celastrol in experimental colitis.Molecular bioSystems · 2016Article
- Alkaline ceramidase 3 deficiency aggravates colitis and colitis-associated tumorigenesis in mice by hyperactivating the innate immune system.Cell death & disease · 2016Article
- Role of acid sphingomyelinase bioactivity in human CD4+ T-cell activation and immune responses.Cell death & disease · 2015Article
- PPARα-dependent exacerbation of experimental colitis by the hypolipidemic drug fenofibrate.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Article
- Inhibition of acid sphingomyelinase by tricyclic antidepressants and analogons.Frontiers in physiology · 2014Review
- Suppression of intestinal inflammation and inflammation-driven colon cancer in mice by dietary sphingomyelin: importance of peroxisome proliferator-activated receptor γ expression.The Journal of nutritional biochemistry · 2011Article
- New insights into the role of fatty acids in the pathogenesis and resolution of inflammatory bowel disease.Inflammatory bowel diseases · 2011Review
- Protective effects of black cumin (Nigella sativa) oil on TNBS-induced experimental colitis in rats.Digestive diseases and sciences · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDietary lipids or pharmacologic modulation of lipid metabolism are potential therapeutic strategies in inflammatory bowel disease (IBD). Therefore, we analysed alterations of bioactive lipids in experimental models of colitis and examined the functional consequence of the second messenger ceramide in inflammatory pathways leading to tissue destruction. METHODOLOGY/PRINCIPAL
findingsChronic colitis was induced by dextran-sulphate-sodium (DSS) or transfer of CD4(+)CD62L(+) cells into RAG1(-/-)-mice. Lipid content of isolated murine intestinal epithelial cells (IEC) was analysed by tandem mass spectrometry. Concentrations of MMP-1 in supernatants of Caco-2-IEC and human intestinal fibroblasts from patients with ulcerative colitis were determined by ELISA. Imipramine was used for pharmacologic inhibition of acid sphingomyelinase (ASM). Ceramide increased by 71% in chronic DSS-induced colitis and by 159% in the transfer model of colitis. Lysophosphatidylcholine (LPC) decreased by 22% in both models. No changes were detected for phosphatidylcholine. Generation of ceramide by exogenous SMase increased MMP-1-protein production of Caco-2-IEC up to 7-fold. Inhibition of ASM completely abolished the induction of MMP-1 by TNF or IL-1beta in Caco-2-IEC and human intestinal fibroblasts. CONCLUSIONS/SIGNIFICANCE: Mucosal inflammation leads to accumulation of ceramide and decrease of LPC in the intestinal epithelium. One aspect of ceramide generation is an increase of MMP-1. Induction of MMP-1 by TNF or IL-1beta is completely blocked by inhibition of ASM with imipramine. Therefore, inhibition of ASM may offer a treatment strategy to reduce MMP-1 expression and tissue destruction in inflammatory conditions.
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