ArticleCellular signalling2010
Prenylation-deficient G protein gamma subunits disrupt GPCR signaling in the zebrafish.
Article in Cellular signalling, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Supracellular contractility in Xenopus embryo epithelia regulated by extracellular ATP and the purinergic receptor P2Y2.Journal of cell science · 2025Article
- Isoliquiritigenin as a Neuronal Radiation Mitigant: Mitigating Radiation-Induced Anhedonia Tendency Targeting Grik3/Grm8/Grin3a via Integrated Proteomics and AI-Driven Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Article
- CaaX-motif adjacent residues control G protein prenylation under suboptimal conditions.bioRxiv : the preprint server for biology · 2023Article
- Subtype-dependent regulation of Gβγ signalling.Cellular signalling · 2021Review
- Regeneration associated transcriptional signature of retinal microglia and macrophages.Scientific reports · 2019Article
- Transducin duplicates in the zebrafish retina and pineal complex: differential specialisation after the teleost tetraploidisation.PloS one · 2015Article
- Identification of differentially expressed genes during development of the zebrafish pineal complex using RNA sequencing.Developmental biology · 2014Article
- Gβγ signaling controls the polarization of zebrafish primordial germ cells by regulating Rac activity.Development (Cambridge, England) · 2012Article
- Central and C-terminal domains of heterotrimeric G protein gamma subunits differentially influence the signaling necessary for primordial germ cell migration.Cellular signalling · 2011Article
- Alkynyl-farnesol reporters for detection of protein S-prenylation in cells.Molecular bioSystems · 2011Article
- Mechanisms guiding primordial germ cell migration: strategies from different organisms.Nature reviews. Molecular cell biology · 2010Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Prenylation of G protein gamma (gamma) subunits is necessary for the membrane localization of heterotrimeric G proteins and for functional heterotrimeric G protein coupled receptor (GPCR) signaling. To evaluate GPCR signaling pathways during development, we injected zebrafish embryos with mRNAs encoding Ggamma subunits mutated so that they can no longer be prenylated. Low-level expression of these prenylation-deficient Ggamma subunits driven either ubiquitously or specifically in the primordial germ cells (PGCs) disrupts GPCR signaling and manifests as a PGC migration defect. This disruption results in a reduction of calcium accumulation in the protrusions of migrating PGCs and a failure of PGCs to directionally migrate. When co-expressed with a prenylation-deficient Ggamma, 8 of the 17 wildtype Ggamma isoforms individually confer the ability to restore calcium accumulation and directional migration. These results suggest that while the Ggamma subunits possess the ability to interact with G Beta (beta) proteins, only a subset of wildtype Ggamma proteins are stable within PGCs and can interact with key signaling components necessary for PGC migration. This in vivo study highlights the functional redundancy of these signaling components and demonstrates that prenylation-deficient Ggamma subunits are an effective tool to investigate the roles of GPCR signaling events during vertebrate development.
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