ArticleNucleic acids research2009
POT1 proteins in green algae and land plants: DNA-binding properties and evidence of co-evolution with telomeric DNA.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Protection of telomeres 1b safeguards the Arabidopsis genome by regulating ROS homeostasis.Nature communications · 2026Article
- GbTRB1 binds telomeric DNA in Ginkgo biloba.BMC plant biology · 2025Article
- Monkeyflower (Mimulus) uncovers the evolutionary basis of the eukaryote telomere sequence variation.PLoS genetics · 2025Article
- Robust Binding Capability and Occasional Gene Loss of Telomere-Binding Proteins Underlying Telomere Evolution in Nematoda.Genome biology and evolution · 2025Article
- Plant telomere biology: The green solution to the end-replication problem.The Plant cell · 2022Article
- Human POT1 unfolds G-quadruplexes by conformational selection.Nucleic acids research · 2020Article
- Recent emergence and extinction of the protection of telomeres 1c gene in Arabidopsis thaliana.Plant cell reports · 2019Article
- Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants.BioNanoScience · 2016Article
- Hypothesis: Paralog Formation from Progenitor Proteins and Paralog Mutagenesis Spur the Rapid Evolution of Telomere Binding Proteins.Frontiers in genetics · 2016Article
- Telomere- and Telomerase-Associated Proteins and Their Functions in the Plant Cell.Frontiers in plant science · 2016Review
- Evolution of the Telomere-Associated Protein POT1a in Arabidopsis thaliana Is Characterized by Positive Selection to Reinforce Protein-Protein Interaction.Molecular biology and evolution · 2015Article
- Tetrahymena Pot2 is a developmentally regulated paralog of Pot1 that localizes to chromosome breakage sites but not to telomeres.Eukaryotic cell · 2014Article
- Extending the model of Arabidopsis telomere length and composition across Brassicaceae.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Article
- Karyotype rearrangements and telomere analysis in Myzuspersicae (Hemiptera, Aphididae) strains collected on Lavandula sp. plants.Comparative cytogenetics · 2014Article
- Signaling of double strand breaks and deprotected telomeres in Arabidopsis.Frontiers in plant science · 2013Review
- Gene networks and chromatin and transcriptional regulation of the phaseolin promoter in Arabidopsis.The Plant cell · 2013Article
- The Oxytricha trifallax macronuclear genome: a complex eukaryotic genome with 16,000 tiny chromosomes.PLoS biology · 2013Article
- Surprises from the chromosome front: lessons from Arabidopsis on telomeres and telomerase.Cold Spring Harbor symposia on quantitative biology · 2012Article
- Selaginella moellendorffii telomeres: conserved and unique features in an ancient land plant lineage.Frontiers in plant science · 2012Article
- QTL Mapping and Candidate Gene Analysis of Telomere Length Control Factors in Maize (Zea mays L.).G3 (Bethesda, Md.) · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Telomeric DNA terminates with a single-stranded 3' G-overhang that in vertebrates and fission yeast is bound by POT1 (Protection Of Telomeres). However, no in vitro telomeric DNA binding is associated with Arabidopsis POT1 paralogs. To further investigate POT1-DNA interaction in plants, we cloned POT1 genes from 11 plant species representing major branches of plant kingdom. Telomeric DNA binding was associated with POT1 proteins from the green alga Ostreococcus lucimarinus and two flowering plants, maize and Asparagus. Site-directed mutagenesis revealed that several residues critical for telomeric DNA recognition in vertebrates are functionally conserved in plant POT1 proteins. However, the plant proteins varied in their minimal DNA-binding sites and nucleotide recognition properties. Green alga POT1 exhibited a strong preference for the canonical plant telomere repeat sequence TTTAGGG with no detectable binding to hexanucleotide telomere repeat TTAGGG found in vertebrates and some plants, including Asparagus. In contrast, POT1 proteins from maize and Asparagus bound TTAGGG repeats with only slightly reduced affinity relative to the TTTAGGG sequence. We conclude that the nucleic acid binding site in plant POT1 proteins is evolving rapidly, and that the recent acquisition of TTAGGG telomere repeats in Asparagus appears to have co-evolved with changes in POT1 DNA sequence recognition.
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