ArticleNucleic acids research2009
Crystal structure of the EndoG/EndoGI complex: mechanism of EndoG inhibition.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- KU80 suppresses endonuclease G activity to preserve genomic integrity.The FEBS journal · 2026Article
- Nucleases and Their Inhibitors: Exploring Biological Roles, Industrial Applications, and Challenges in Heterologous Expression.Biotechnology journal · 2026Review
- Human Endonuclease G Preferentially Cleaves Oxidatively Damaged DNA.Biochemistry · 2026Article
- Discovery of an Endonuclease G-inhibitory Ku80-peptide protecting against leukemogenic rearrangements at the MLL breakpoint cluster.Nature communications · 2026Article
- Identification and characterization of a dsRNA-degrading nuclease CmdsRNase2 influencing RNAi efficiency in the rice leaffolder Cnaphalocrocis medinalis.Scientific reports · 2025Article
- Mitochondrial exonuclease EXOG supports DNA integrity by the removal of single-stranded DNA flaps.Nucleic acids research · 2025Article
- Identification and Characterization of a Double-Stranded RNA Degrading Nuclease Influencing RNAi Efficiency in the Rice Leaf FolderInternational journal of molecular sciences · 2022Article
- Characterization of a cell death-inducing endonuclease-like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae).Pest management science · 2021Article
- Role of Bacterial and Host DNases on Host-Pathogen Interaction duringInternational journal of molecular sciences · 2020Article
- Structural adaptation of vertebrate endonuclease G for 5-hydroxymethylcytosine recognition and function.Nucleic acids research · 2020Article
- Anti-ROR1 scFv-EndoG as a Novel Anti-Cancer Therapeutic DrugAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Immunology and Genetic ofJournal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2018Review
- Systematic classification of the His-Me finger superfamily.Nucleic acids research · 2017Article
- Structure-based domain assignment in Leishmania infantum EndoG: characterization of a pH-dependent regulatory switch and a C-terminal extension that largely dictates DNA substrate preferences.Nucleic acids research · 2017Article
- Dnases in health and disease.Developmental biology · 2017Review
- A domain in human EXOG converts apoptotic endonuclease to DNA-repair exonuclease.Nature communications · 2017Article
- Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis.PloS one · 2017Article
- Crystal structure of endonuclease G in complex with DNA reveals how it nonspecifically degrades DNA as a homodimer.Nucleic acids research · 2016Article
- Structural characterization of the virulence factor nuclease A from Streptococcus agalactiae.Acta crystallographica. Section D, Biological crystallography · 2014Article
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Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
EndoG is a ubiquitous nuclease that is translocated into the nucleus during apoptosis to participate in DNA degradation. The enzyme cleaves double- and single-stranded DNA and RNA. Related nucleases are found in eukaryotes and prokaryotes, which have evolved sophisticated mechanisms for genome protection against self-antagonizing nuclease activity. Common mechanisms of inhibition are secretion, sequestration into a separate cellular compartment or by binding to protein inhibitors. Although EndoG is silenced by compartmentalization into the mitochondrial intermembrane space, a nucleus-localized protein inhibitor protects cellular polynucleotides from degradation by stray EndoG under non-apoptotic conditions in Drosophila. Here, we report the first three-dimensional structure of EndoG in complex with its inhibitor EndoGI. Although the mechanism of inhibition is reminiscent of bacterial protein inhibitors, EndoGI has evolved independently from a generic protein-protein interaction module. EndoGI is a two-domain protein that binds the active sites of two monomers of EndoG, with EndoG being sandwiched between EndoGI. Since the amino acid sequences of eukaryotic EndoG homologues are highly conserved, this model is valid for eukaryotic dimeric EndoG in general. The structure indicates that the two active sites of EndoG occupy the most remote spatial position possible at the molecular surface and a concerted substrate processing is unlikely.
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Registered trials
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