ArticleNucleic acids research2009
The single polypeptide restriction-modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- ENDO-Pore: high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing.Nucleic acids research · 2021Article
- DNA-mediated coupling of ATPase, translocase and nuclease activities of a Type ISP restriction-modification enzyme.Nucleic acids research · 2020Article
- CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases.Nucleic acids research · 2017Article
- Novel m4C modification in type I restriction-modification systems.Nucleic acids research · 2016Article
- Structural insights into DNA sequence recognition by Type ISP restriction-modification enzymes.Nucleic acids research · 2016Article
- Mapping DNA cleavage by the Type ISP restriction-modification enzymes following long-range communication between DNA sites in different orientations.Nucleic acids research · 2015Article
- Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes.Nature chemical biology · 2015Article
- Type I restriction enzymes and their relatives.Nucleic acids research · 2014Review
- The Type ISP Restriction-Modification enzymes LlaBIII and LlaGI use a translocation-collision mechanism to cleave non-specific DNA distant from their recognition sites.Nucleic acids research · 2013Article
- DNA cleavage by Type ISP Restriction-Modification enzymes is initially targeted to the 3'-5' strand.Nucleic acids research · 2013Article
- Sequence-specific assembly of FtsK hexamers establishes directional translocation on DNA.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Type III restriction enzymes cleave DNA by long-range interaction between sites in both head-to-head and tail-to-tail inverted repeat.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- An Mrr-family nuclease motif in the single polypeptide restriction-modification enzyme LlaGI.Nucleic acids research · 2009Article
- DNA cleavage and methylation specificity of the single polypeptide restriction-modification enzyme LlaGI.Nucleic acids research · 2009Article
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3 authors.
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Abstract
To cleave DNA, the single polypeptide restriction-modification enzyme LlaGI must communicate between a pair of indirectly repeated recognition sites. We demonstrate that this communication occurs by a 1-dimensional route, namely unidirectional dsDNA loop translocation rightward of the specific recognition sequence 5'-CTnGAyG-3' as written (where n is either A, G, C or T and y is either C or T). Motion across thousands of base pairs is catalysed by the helicase domain and requires the hydrolysis of 1.5-2 ATP per base pair. DNA loop extrusion is accompanied by changes in DNA twist consistent with the motor following the helical pitch of the polynucleotide track. LlaGI is therefore an example of a polypeptide that is a completely self-contained, multi-functional molecular machine.
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