ArticleApplied and environmental microbiology2009
Molecular characterization of potential nitrogen fixation by anaerobic methane-oxidizing archaea in the methane seep sediments at the number 8 Kumano Knoll in the Kumano Basin, offshore of Japan.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- The hidden potential of archaea in carbon and nitrogen cycling in agricultural soils: a review.Frontiers in microbiology · 2026Review
- Non-cyanobacterial diazotrophs: global diversity, distribution, ecophysiology, and activity in marine waters.FEMS microbiology reviews · 2023Review
- Article
- Phylogenetically and catabolically diverse diazotrophs reside in deep-sea cold seep sediments.Nature communications · 2022Article
- Understanding Interaction Patterns within Deep-Sea Microbial Communities and Their Potential Applications.Marine drugs · 2022Review
- Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough.Frontiers in microbiology · 2022Article
- Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.PLoS biology · 2022Review
- Abundance and diversity of diazotrophs in the surface sediments of Kongsfjorden, an Arctic fjord.World journal of microbiology & biotechnology · 2021Article
- Dual energy metabolism of the Campylobacterota endosymbiont in the chemosynthetic snail Alviniconcha marisindica.The ISME journal · 2020Article
- The Impact of Methane on Microbial Communities at Marine Arctic Gas Hydrate Bearing Sediment.Frontiers in microbiology · 2020Article
- Subsurface Microbial Hydrogen Cycling: Natural Occurrence and Implications for Industry.Microorganisms · 2019Review
- Rates and Microbial Players of Iron-Driven Anaerobic Oxidation of Methane in Methanic Marine Sediments.Frontiers in microbiology · 2019Article
- Nitrogen Fixation in Thermophilic Chemosynthetic Microbial Communities Depending on Hydrogen, Sulfate, and Carbon Dioxide.Microbes and environments · 2018Article
- Ecological Energetic Perspectives on Responses of Nitrogen-Transforming Chemolithoautotrophic Microbiota to Changes in the Marine Environment.Frontiers in microbiology · 2017Review
- Article
- Activity and interactions of methane seep microorganisms assessed by parallel transcription and FISH-NanoSIMS analyses.The ISME journal · 2016Article
- Environmental Conditions Outweigh Geographical Contiguity in Determining the Similarity of nifH-Harboring Microbial Communities in Sediments of Two Disconnected Marginal Seas.Frontiers in microbiology · 2016Article
- Species Divergence vs. Functional Convergence Characterizes Crude Oil Microbial Community Assembly.Frontiers in microbiology · 2016Article
- Niche differentiation of bacterial communities at a millimeter scale in Shark Bay microbial mats.Scientific reports · 2015Article
- Microbial community changes along the active seepage site of one cold seep in the Red Sea.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potential for microbial nitrogen fixation in the anoxic methane seep sediments in a mud volcano, the number 8 Kumano Knoll, was characterized by molecular phylogenetic analyses. A total of 111 of the nifH (a gene coding a nitrogen fixation enzyme, Fe protein) clones were obtained from different depths of the core sediments, and the phylogenetic analysis of the clones indicated the genetic diversity of nifH genes. The predominant group detected (methane seep group 2), representing 74% of clonal abundance, was phylogenetically related to the nifH sequences obtained from the Methanosarcina species but was most closely related to the nifH sequences potentially derived from the anoxic methanotrophic archaea (ANME-2 archaea). The recovery of the nif gene clusters including the nifH sequences of the methane seep group 2 and the subsequent reverse transcription-PCR detection of the nifD and nifH genes strongly suggested that the genetic components of the gene clusters would be operative for the in situ assimilation of molecular nitrogen (N(2)) by the host microorganisms. DNA-based quantitative PCR of the archaeal 16S rRNA gene, the group-specific mcrA (a gene encoding the methyl-coenzyme M reductase alpha subunit) gene, and the nifD and nifH genes demonstrated the similar distribution patterns of the archaeal 16S rRNA gene, the mcrA groups c-d and e, and the nifD and nifH genes through the core sediments. These results supported the idea that the anoxic methanotrophic archaea ANME-2c could be the microorganisms hosting the nif gene clusters and could play an important role in not only the in situ carbon (methane) cycle but also the nitrogen cycle in subseafloor sediments.
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