ArticleThe Journal of biological chemistry2009
Membrane rafts are involved in intracellular miconazole accumulation in yeast cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- yEvo: experimental evolution in high school classrooms selects for novel mutations that impact clotrimazole resistance in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2022Article
- Coordinated Regulation of Membrane Homeostasis and Drug Accumulation by Novel Kinase STK-17 in Response to Antifungal Azole Treatment.Microbiology spectrum · 2022Article
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- Stimulation of superoxide production increases fungicidal action of miconazole against Candida albicans biofilms.Scientific reports · 2016Article
- De-repression of CSP-1 activates adaptive responses to antifungal azoles.Scientific reports · 2016Article
- Iterative carotenogenic screens identify combinations of yeast gene deletions that enhance sclareol production.Microbial cell factories · 2015Article
- The yeast sphingolipid signaling landscape.Chemistry and physics of lipids · 2014Review
- Phytosphingosine-1-phosphate is a signaling molecule involved in miconazole resistance in sessile Candida albicans cells.Antimicrobial agents and chemotherapy · 2012Article
- The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans.Molecular microbiology · 2012Article
- Arv1 lipid transporter function is conserved between pathogenic and nonpathogenic fungi.Fungal genetics and biology : FG & B · 2012Article
- Lipid raft involvement in yeast cell growth and death.Frontiers in oncology · 2012Article
- Superoxide dismutases are involved in Candida albicans biofilm persistence against miconazole.Antimicrobial agents and chemotherapy · 2011Article
- Miconazole induces fungistasis and increases killing of Candida albicans subjected to photodynamic therapy.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
Abstract
Azoles inhibit ergosterol biosynthesis, resulting in ergosterol depletion and accumulation of toxic 14alpha-methylated sterols in membranes of susceptible yeast. We demonstrated previously that miconazole induces actin cytoskeleton stabilization in Saccharomyces cerevisiae prior to induction of reactive oxygen species, pointing to an ancillary mode of action. Using a genome-wide agar-based screening, we demonstrate in this study that S. cerevisiae mutants affected in sphingolipid and ergosterol biosynthesis, namely ipt1, sur1, skn1, and erg3 deletion mutants, are miconazole-resistant, suggesting an involvement of membrane rafts in its mode of action. This is supported by the antagonizing effect of membrane raft-disturbing compounds on miconazole antifungal activity as well as on miconazole-induced actin cytoskeleton stabilization and reactive oxygen species accumulation. These antagonizing effects point to a primary role for membrane rafts in miconazole antifungal activity. We further show that this primary role of membrane rafts in miconazole action consists of mediating intracellular accumulation of miconazole in yeast cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.