ArticleJournal of molecular biology2009
Residues gating the periplasmic pathway of LacY.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- The ins and outs of vesicular monoamine transporters.The Journal of general physiology · 2018Review
- Identification of PLXDC1 and PLXDC2 as the transmembrane receptors for the multifunctional factor PEDF.eLife · 2014Article
- Galactoside-binding site in LacY.Biochemistry · 2014Article
- Structure of sugar-bound LacY.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Identification of conformationally sensitive residues essential for inhibition of vesicular monoamine transport by the noncompetitive inhibitor tetrabenazine.The Journal of biological chemistry · 2013Article
- The major facilitator superfamily (MFS) revisited.The FEBS journal · 2012Article
- Basic residues R260 and K357 affect the conformational dynamics of the major facilitator superfamily multidrug transporter LmrP.PloS one · 2012Article
- Lactose permease and the alternating access mechanism.Biochemistry · 2011Article
- An early event in the transport mechanism of LacY protein: interaction between helices V and I.The Journal of biological chemistry · 2011Article
- Random mutagenesis of the prokaryotic peptide transporter YdgR identifies potential periplasmic gating residues.The Journal of biological chemistry · 2011Article
- Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport.Biochemistry · 2011Article
- The alternating access transport mechanism in LacY.The Journal of membrane biology · 2011Review
- Probing the periplasmic-open state of lactose permease in response to sugar binding and proton translocation.Journal of molecular biology · 2010Article
- Using student-generated UV-induced Escherichia coli mutants in a directed inquiry undergraduate genetics laboratory.Genetics · 2010Article
- Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Helix dynamics in LacY: helices II and IV.Journal of molecular biology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
X-ray crystal structures of LacY (lactose permease of Escherichia coli) exhibit a large cytoplasmic cavity containing the residues involved in sugar binding and H(+) translocation at the apex and a tightly packed side facing the periplasm. However, biochemical and biophysical evidence provide a strong indication that a hydrophilic pathway opens on the external surface of LacY with closing of the cytoplasmic side upon sugar binding. Thus, an alternating-access mechanism in which sugar- and H(+)-binding sites at the approximate middle of the molecule are alternatively exposed to either side of the membrane is likely to underlie LacY-catalyzed sugar/H(+) symport. To further investigate periplasmic opening, we replaced paired residues on the tightly packed periplasmic side of LacY with Cys, and the effect of cross-linking was studied by testing the accessibility/reactivity of Cys148 with the elongated ( approximately 29 A), impermeant hydrophilic reagent maleimide-PEG2-biotin. When the paired-Cys mutant Ile40-->Cys/Asn245-->Cys containing native Cys148 is oxidized to form a disulfide bond, the reactivity of Cys148 is markedly inhibited. Moreover, the reactivity of Cys148 in this mutant increases with the length of the cross-linking agent. In contrast, maleimide-PEG2-biotin reactivity of Cys148 is unaffected by oxidation of two other paired-Cys mutants at the mouth of the periplasmic cavity. The data indicate that residues Ile40 and Asn245 play a primary role in gating the periplasmic cavity and provide further support for the alternating-access model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.