ArticleGenome biology2009
Comparative genome and phenotypic analysis of Clostridium difficile 027 strains provides insight into the evolution of a hypervirulent bacterium.
Article in Genome biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 309 papers, 1 of them a synthesis that pooled it.
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Who cites it
309 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nonmammalian models to study Clostridioides difficile infection; a systematic review.Anaerobe · 2023Pooled it
- Antimicrobial susceptibility and ribotypes of Clostridium difficile isolates from a Phase 2 clinical trial of ridinilazole (SMT19969) and vancomycin.The Journal of antimicrobial chemotherapy · 2018Trial
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- A programmed reset mechanism primesScience advances · 2026Article
- 1-Kestose as a Candidate Precision Bioactive Component: From GH32-Dependent Gut Microbiota Regulation to Human Health Enhancement.Foods (Basel, Switzerland) · 2026Review
- Coupling Far-Western Blotting with Peptide Microarrays Reveals Novel E-Cadherin Spore-Surface Ligands inJournal of proteome research · 2026Article
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- Phylogenomic analysis of the collagen-like BclA proteins inApplied and environmental microbiology · 2026Article
- CenIR, an essential BlaIR-family regulatory system inbioRxiv : the preprint server for biology · 2026Article
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- Comparative genomic analysis of Clostridioides difficile strains in Mexico: insights into virulence and resistance.Access microbiology · 2026Article
- Differences in virulence and drug resistance betweenVirulence · 2025Article
- Analysis of essential genes inJournal of bacteriology · 2025Article
- History and Evolution of the HypervirulentMicroorganisms · 2025Review
- Deciphering the RNA-based regulation mechanism of the phage-encoded AbiF system in Clostridioides difficile.PLoS genetics · 2025Article
- Article
- Phenotypic analysis of variousApplied and environmental microbiology · 2025Article
- Analysis of Essential Genes inbioRxiv : the preprint server for biology · 2025Article
249 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors.
Funding
Abstract
backgroundThe continued rise of Clostridium difficile infections worldwide has been accompanied by the rapid emergence of a highly virulent clone designated PCR-ribotype 027. To understand more about the evolution of this virulent clone, we made a three-way genomic and phenotypic comparison of an 'historic' non-epidemic 027 C. difficile (CD196), a recent epidemic and hypervirulent 027 (R20291) and a previously sequenced PCR-ribotype 012 strain (630).
resultsAlthough the genomes are highly conserved, the 027 genomes have 234 additional genes compared to 630, which may contribute to the distinct phenotypic differences we observe between these strains relating to motility, antibiotic resistance and toxicity. The epidemic 027 strain has five unique genetic regions, absent from both the non-epidemic 027 and strain 630, which include a novel phage island, a two component regulatory system and transcriptional regulators.
conclusionsA comparison of a series of 027 isolates showed that some of these genes appeared to have been gained by 027 strains over the past two decades. This study provides genetic markers for the identification of 027 strains and offers a unique opportunity to explain the recent emergence of a hypervirulent bacterium.
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