ArticlePloS one2009
A screen for spore wall permeability mutants identifies a secreted protease required for proper spore wall assembly.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular and Biophysical Perspectives on Dormancy Breaking: Lessons from Yeast Spore.Biomolecules · 2025Review
- Remodeling of the secretory pathway is coordinated withiScience · 2024Article
- Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2021Article
- A fluorescence-based yeast sensor for monitoring acetic acid.Engineering in life sciences · 2021Article
- Article
- Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents.Microbial cell factories · 2019Article
- Transcriptome profiling of Issatchenkia orientalis under ethanol stress.AMB Express · 2018Article
- Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation.Molecular biology of the cell · 2017Article
- A Novel Assay Reveals a Maturation Process during Ascospore Wall Formation.Journal of fungi (Basel, Switzerland) · 2017Article
- Evidence for ESCRT- and clathrin-dependent microautophagy.The Journal of cell biology · 2017Article
- Article
- Production of encapsulated creatinase using yeast spores.Bioengineered · 2017Article
- Chitosan Mediates Germling Adhesion in Magnaporthe oryzae and Is Required for Surface Sensing and Germling Morphogenesis.PLoS pathogens · 2016Article
- Assembly, molecular organization, and membrane-binding properties of development-specific septins.The Journal of cell biology · 2016Article
- Roles of septins in prospore membrane morphogenesis and spore wall assembly in Saccharomyces cerevisiae.Molecular biology of the cell · 2016Article
- Whole-genome de novo sequencing, combined with RNA-Seq analysis, reveals unique genome and physiological features of the amylolytic yeastBiotechnology for biofuels · 2016Article
- Bioconversion of D-glucose to D-psicose with immobilized D-xylose isomerase and D-psicose 3-epimerase on Saccharomyces cerevisiae spores.Journal of industrial microbiology & biotechnology · 2015Article
- Transcriptome structure variability in Saccharomyces cerevisiae strains determined with a newly developed assembly software.BMC genomics · 2014Article
- Applied usage of yeast spores as chitosan beads.Applied and environmental microbiology · 2014Article
- Use of yeast spores for microencapsulation of enzymes.Applied and environmental microbiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ascospores of Saccharomyces cerevisiae are surrounded by a complex wall that protects the spores from environmental stresses. The outermost layer of the spore wall is composed of a polymer that contains the cross-linked amino acid dityrosine. This dityrosine layer is important for stress resistance of the spore. This work reports that the dityrosine layer acts as a barrier blocking the diffusion of soluble proteins out of the spore wall into the cytoplasm of the ascus. Diffusion of a fluorescent protein out of the spore wall was used as an assay to screen for mutants affecting spore wall permeability. One of the genes identified in this screen, OSW3 (RRT12/YCR045c), encodes a subtilisin-family protease localized to the spore wall. Mutation of the active site serine of Osw3 results in spores with permeable walls, indicating that the catalytic activity of Osw3 is necessary for proper construction of the dityrosine layer. These results indicate that dityrosine promotes stress resistance by acting as a protective shell around the spore. OSW3 and other OSW genes identified in this screen are strong candidates to encode enzymes involved in assembly of this protective dityrosine coat.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.