ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009
Human duodenum responses to vitamin D metabolites of TRPV6 and other genes involved in calcium absorption.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 91 citations in OpenAlex.
- Stimulation of intestinal calcium absorption by orally administrated vitamin D3 compounds: a prospective open-label randomized trial in osteoporosis.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2018Trial
- Trial
- Vitamin D is glucoprotective in aging males but not females.The Journal of steroid biochemistry and molecular biology · 2026Article
- Article
- The endocannabinoid system in bovine tissues: characterization of transcript abundance in the growing Holstein steer.BMC veterinary research · 2024Article
- Review
- Evaluation of the outcome of a proposed more physiological bypass surgery technique in morbid obesity: Long term 3 years follows up.Annals of medicine and surgery (2012) · 2022Article
- Calcium selective channel TRPV6: Structure, function, and implications in health and disease.Gene · 2022Review
- Structure and function of the calcium-selective TRP channel TRPV6.The Journal of physiology · 2021Review
- Analysis of 1,25-Dihydroxyvitamin DMolecular and cellular biology · 2020Article
- Intestinal CaWorld journal of gastroenterology · 2020Review
- Oral 25-Hydroxycholecalciferol Acts as an Agonist in the Duodenum of Mice and as Modeled in Cultured Human HT-29 and Caco2 Cells.The Journal of nutrition · 2020Article
- TRPV6 as A Target for Cancer Therapy.Journal of Cancer · 2020Review
- Preoperative vitamin D deficiency is a risk factor for postoperative hypocalcemia in patients undergoing total thyroidectomy: retrospective cohort study.Sao Paulo medical journal = Revista paulista de medicina · 2019Article
- Hepatic Transport of 25-Hydroxyvitamin DDrug metabolism and disposition: the biological fate of chemicals · 2018Article
- Association of Sun Exposure, Skin Colour and Body Mass Index with Vitamin D Status in Individuals Who Are Morbidly Obese.Nutrients · 2017Article
- Association between Serum 25-Hydroxy-Vitamin D and Aggressive Prostate Cancer in African American Men.Nutrients · 2016Article
- PTH and Vitamin D.Comprehensive Physiology · 2016Review
- CYP24 inhibition as a therapeutic target in FGF23-mediated renal phosphate wasting disorders.The Journal of clinical investigation · 2016Article
- Molecular aspects of intestinal calcium absorption.World journal of gastroenterology · 2015Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Calcium absorption by the intestine is necessary for bone mineralization. Much has been learned about this process and the role of vitamin D metabolites in gene transcription from animal studies, but the molecular mechanisms in humans are less well understood. We have used samples of normal human duodenal mucosa, obtained at endoscopy, to investigate the effects of the vitamin D metabolites, 1alpha-dihydroxycholecalciferol [1,25(OH)(2)D(3)] and 25-hydroxycholecalciferol (25OHD), on transcripts on genes involved in calcium absorption and vitamin D metabolism. TRPV6 transcripts were significantly higher after incubation for 6 h with 1,25(OH)(2)D(3) (10(-9) mol/l) than after control incubations (median difference 3.1-fold, P < 0.001). Unexpectedly, TRPV6 expression was also higher (2.4-fold, P < 0.02) after incubation with 25OHD (10(-7) mol/l). Transcripts for the calcium-ATPase, PMCA1, were significantly higher with 1,25(OH)(2)D(3); CYP24 transcripts were reliably detected after incubation with either metabolite, but calbindin-D9k transcripts were unaffected. The response of TRPV6 to 25OHD and the expression of transcripts for CYP27B1, the 25OHD-1alpha-hydroxylase, were significantly correlated (r = 0.82, P < 0.02). Basal duodenal expression of TRPV6 and CYP27B1 were significantly associated (r = 0.72, P < 0.001) in a separate previously reported series of subjects. Multiple regression analysis of the associations with basal duodenal TRPV6 expression identified CYP27B1 expression and serum 1,25(OH)(2)D as major factors. Expression of the CYP27B1 protein was demonstrated immunohistochemically in duodenal mucosa. This study has shown that human duodenal TRPV6, PMCA1, and CYP24 transcripts respond rapidly to 1,25(OH)(2)D(3) and provides evidence suggesting that local duodenal production of 1,25(OH)(2)D(3) by 25OHD-1alpha-hydroxylase may have a role in human calcium absorption.
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