ArticleThe Journal of biological chemistry2009
Interactions between EB1 and microtubules: dramatic effect of affinity tags and evidence for cooperative behavior.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- Dysregulation of the Tau-Microtubule-End-Binding Protein Axis in Alzheimer's Disease and Related Tauopathies.International journal of molecular sciences · 2026Review
- The Effect of Protein Tagging on Aggregation and Phase Separation.Journal of cellular biochemistry · 2026Review
- Article
- Microtubules in Martini: Parameterizing a heterogeneous elastic-network towards a mechanically accurate microtubule.PNAS nexus · 2025Article
- Terminator: A Software Package for Fast and Local Optimization of His-Tag Placement for Protein Affinity Purification.ACS bio & med chem Au · 2025Article
- Glutamylation is a negative regulator of microtubule growth.Molecular biology of the cell · 2023Article
- Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outside.Science advances · 2022Article
- Insights into the mechanism of action of the arbitrium communication system in SPbeta phages.Nature communications · 2022Article
- Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells.PLoS genetics · 2020Article
- Structural state recognition facilitates tip tracking of EB1 at growing microtubule ends.eLife · 2019Article
- Native kinesin-1 does not bind preferentially to GTP-tubulin-rich microtubules in vitro.Cytoskeleton (Hoboken, N.J.) · 2017Article
- Interactions between the Microtubule Binding Protein EB1 and F-Actin.Journal of molecular biology · 2016Article
- Self protein-protein interactions are involved in TPPP/p25 mediated microtubule bundling.Scientific reports · 2015Article
- Article
- Disease mutations in desmoplakin inhibit Cx43 membrane targeting mediated by desmoplakin-EB1 interactions.The Journal of cell biology · 2014Article
- An improved method and cost effective strategy for soluble expression and purification of human N-myristoyltransferase 1 in E. coli.Molecular and cellular biochemistry · 2014Article
- Ectopic A-lattice seams destabilize microtubules.Nature communications · 2014Article
- Direct regulation of microtubule dynamics by KIF17 motor and tail domains.The Journal of biological chemistry · 2013Article
- Dynactin subunit p150(Glued) is a neuron-specific anti-catastrophe factor.PLoS biology · 2013Article
- Biochemical evidence that human EB1 does not bind preferentially to the microtubule seam.Cytoskeleton (Hoboken, N.J.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Plus end tracking proteins (+TIPs) are a unique group of microtubule binding proteins that dynamically track microtubule (MT) plus ends. EB1 is a highly conserved +TIP with a fundamental role in MT dynamics, but it remains poorly understood in part because reported EB1 activities have differed considerably. One reason for this inconsistency could be the variable presence of affinity tags used for EB1 purification. To address this question and establish the activity of native EB1, we have measured the MT binding and tubulin polymerization activities of untagged EB1 and EB1 fragments and compared them with those of His-tagged EB1 proteins. We found that N-terminal His tags directly influence the interaction between EB1 and MTs, significantly increasing both affinity and activity, and that small amounts of His-tagged proteins act synergistically with larger amounts of untagged proteins. Moreover, the binding ratio between EB1 and tubulin can exceed 1:1, and EB1-MT binding curves do not fit simple binding models. These observations demonstrate that EB1 binding is not limited to the MT seam, and they suggest that EB1 binds cooperatively to MTs. Finally, we found that removal of tubulin C-terminal tails significantly reduces EB1 binding, indicating that EB1-tubulin interactions are mediated in part by the same tubulin acidic tails utilized by other MAPs. These binding relationships are important for helping to elucidate the complex of proteins at the MT tip.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.