ArticleAntonie van Leeuwenhoek2009
Evaluating amplified rDNA restriction analysis assay for identification of bacterial communities.
Article in Antonie van Leeuwenhoek, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Current Methods for Reliable Identification of Species in theMicroorganisms · 2025Review
- Research Progresses on the Function and Detection Methods of Insect Gut Microbes.Microorganisms · 2023Review
- Impact of Cultivation Substrate and Microbial Community on Improving Mushroom Productivity: A Review.Biology · 2022Review
- Tyrosinase and laccase-producing Bacillus aryabhattai TFG5 and its role in the polymerization of phenols.BMC microbiology · 2021Article
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
- A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information.BMC microbiology · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amplified ribosomal DNA restriction analysis (ARDRA) and restriction fragment length polymorphism were originally used for strain typing and for screening clone libraries to identify phylogenetic clusters within a microbial community. Here we used ARDRA as a model to examine the capacity of restriction-based techniques for clone identification, and the possibility of deriving phylogenetic information from ARDRA-based dendrograms. ARDRA was performed in silico on 48,759 sequences from the Ribosomal Database Project, and it was found that the fragmentation profiles were not necessarily unique for each sequence in the database, resulting in different species sharing fragmentation profiles. Although ARDRA-based clusters separated clones into different genera, these phylogenetic clusters did not overlap with trees constructed according to sequence alignment,calling into question the intra-genus ARDRA based phylogeny. It is thus suggested that the prediction power of ARDRA clusters in identifying clone phylogeny be regarded with caution.
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Registered trials
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