ArticleMolecular biology of the cell2009
Yeast Sec1p functions before and after vesicle docking.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- The small GTPases FoRab5, FoRab7, and FoRab8 regulate vesicle transport to modulate vegetative development and pathogenicity inFrontiers in microbiology · 2025Article
- Emerging Roles of Exocyst Complex in Fungi: A Review.Journal of fungi (Basel, Switzerland) · 2024Review
- High-resolution secretory timeline from vesicle formation at the Golgi to fusion at the plasma membrane ineLife · 2022Article
- Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris.Biotechnology for biofuels · 2021Article
- Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris.Microbial cell factories · 2021Article
- Roles of Mso1 and the SM protein Sec1 in efficient vesicle fusion during fission yeast cytokinesis.Molecular biology of the cell · 2020Article
- Article
- Munc18a clusters SNARE-bearing liposomes prior toThe Biochemical journal · 2017Article
- Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris.Scientific reports · 2017Article
- Protein structure and phenotypic analysis of pathogenic and population missense variants inMolecular genetics & genomic medicine · 2017Article
- SM protein Munc18-2 facilitates transition of Syntaxin 11-mediated lipid mixing to complete fusion for T-lymphocyte cytotoxicity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion.Nature communications · 2017Article
- Engineering vesicle trafficking improves the extracellular activity and surface display efficiency of cellulases inBiotechnology for biofuels · 2017Article
- The Secret Life of Tethers: The Role of Tethering Factors in SNARE Complex Regulation.Frontiers in cell and developmental biology · 2016Review
- Subunit connectivity, assembly determinants and architecture of the yeast exocyst complex.Nature structural & molecular biology · 2016Article
- Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes.Human molecular genetics · 2013Article
- Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Sec1/Munc18 protein Vps33 binds to SNARE domains and the quaternary SNARE complex.Molecular biology of the cell · 2012Article
- Munc18-1 mutations that strongly impair SNARE-complex binding support normal synaptic transmission.The EMBO journal · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Sec1/Munc18 (SM) proteins bind cognate soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes and stimulate vesicle membrane fusion. Before fusion, vesicles are docked to specific target membranes. Regulation of vesicle docking is attributed to some but not all SM proteins, suggesting specialization of this earlier function. Yeast Sec1p seems to function only after vesicles are docked and SNARE complexes are assembled. Here, we show that yeast Sec1p is required before and after SNARE complex assembly, in support of general requirements for SM proteins in both vesicle docking and fusion. Two classes of sec1 mutants were isolated. Class A mutants are tightly blocked in cell growth and secretion at a step before SNARE complex assembly. Class B mutants have a SNARE complex binding defect, with a range in severity of cell growth and secretion defects. Mapping the mutations onto an SM protein structure implicates a peripheral bundle of helices for the early, docking function and a deep groove, opposite the syntaxin-binding cleft on nSec1/Munc-18, for the interaction between Sec1p and the exocytic SNARE complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.