ArticleThe Journal of biological chemistry2009
Ribosomal protein S12 and aminoglycoside antibiotics modulate A-site mRNA cleavage and transfer-messenger RNA activity in Escherichia coli.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Antibiotic resistance increases evolvability and maximizes opportunities across fitness landscapes.Frontiers in microbiology · 2026Article
- Evolutionary trajectory of bacterial resistance to antibiotics and antimicrobial peptides inmSystems · 2025Article
- The Application of Regulatory Cascades inFrontiers in microbiology · 2020Review
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- The fitness costs of antibiotic resistance mutations.Evolutionary applications · 2015Review
- Article
- Streptomycin Induced Stress Response in Salmonella enterica Serovar Typhimurium Shows Distinct Colony Scatter Signature.PloS one · 2015Article
- Restrictive Streptomycin Resistance Mutations Decrease the Formation of Attaching and Effacing Lesions in Escherichia coli O157:H7 Strains.Antimicrobial agents and chemotherapy · 2013Article
- A novel family of toxin/antitoxin proteins in Bacillus species.FEBS letters · 2012Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- The role of SmpB and the ribosomal decoding center in licensing tmRNA entry into stalled ribosomes.RNA (New York, N.Y.) · 2011Article
- tmRNA regulates synthesis of the ArfA ribosome rescue factor.Molecular microbiology · 2011Article
- Genome engineering using targeted oligonucleotide libraries and functional selection.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Deletion of the RluD pseudouridine synthase promotes SsrA peptide tagging of ribosomal protein S7.Molecular microbiology · 2011Article
- Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Translational pausing in Escherichia coli can lead to mRNA cleavage within the ribosomal A-site. A-site mRNA cleavage is thought to facilitate transfer-messenger RNA (tmRNA).SmpB- mediated recycling of stalled ribosome complexes. Here, we demonstrate that the aminoglycosides paromomycin and streptomycin inhibit A-site cleavage of stop codons during inefficient translation termination. Aminoglycosides also induced stop codon read-through, suggesting that these antibiotics alleviate ribosome pausing during termination. Streptomycin did not inhibit A-site cleavage in rpsL mutants, which express streptomycin-resistant variants of ribosomal protein S12. However, rpsL strains exhibited reduced A-site mRNA cleavage compared with rpsL(+) cells. Additionally, tmRNA.SmpB-mediated SsrA peptide tagging was significantly reduced in several rpsL strains but could be fully restored in a subset of mutants when treated with streptomycin. The streptomycin-dependent rpsL(P90K) mutant also showed significantly lower levels of A-site cleavage and tmRNA.SmpB activity. Mutations in rpsD (encoding ribosomal protein S4), which suppressed streptomycin dependence, were able to partially restore A-site cleavage to rpsL(P90K) cells but failed to increase tmRNA.SmpB activity. Taken together, these results show that perturbations to A-site structure and function modulate A-site mRNA cleavage and tmRNA.SmpB activity. We propose that tmRNA.SmpB binds to streptomycin-resistant rpsL ribosomes less efficiently, leading to a partial loss of ribosome rescue function in these mutants.
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