ArticleInternational journal of cardiology2011
Differentiation induction of mouse embryonic stem cells into sinus node-like cells by suramin.
Article in International journal of cardiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
- Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes.Biology open · 2023Article
- Uniformly-aligned gelatin/polycaprolactone fibers promote proliferation in adipose-derived stem cells and have distinct effects on cardiac cell differentiation.International journal of clinical and experimental pathology · 2021Article
- Molecular Characterization of Embryonic Stem Cell-Derived Cardiac Neural Crest-Like Cells Revealed a Spatiotemporal Expression of an Mlc-3 Isoform.International journal of stem cells · 2020Article
- Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells.Stem cells (Dayton, Ohio) · 2020Article
- Tbx18-dependent differentiation of brown adipose tissue-derived stem cells toward cardiac pacemaker cells.Molecular and cellular biochemistry · 2017Article
- Involvement of Rictor/mTORC2 in cardiomyocyte differentiation of mouse embryonic stem cellsInternational journal of biological sciences · 2017Article
- Overexpression of Map3k7 activates sinoatrial node-like differentiation in mouse ES-derived cardiomyocytes.PloS one · 2017Article
- Efficient Generation of Cardiac Purkinje Cells from ESCs by Activating cAMP Signaling.Stem cell reports · 2015Article
- Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.Stem cells (Dayton, Ohio) · 2015Article
- SHOX2 overexpression favors differentiation of embryonic stem cells into cardiac pacemaker cells, improving biological pacing ability.Stem cell reports · 2015Article
- Programming and isolation of highly pure physiologically and pharmacologically functional sinus-nodal bodies from pluripotent stem cells.Stem cell reports · 2014Article
- A combination treatment with DNA methyltransferase inhibitors and suramin decreases invasiveness of breast cancer cells.Breast cancer research and treatment · 2014Article
- Time-dependent regulation of neuregulin-1β/ErbB/ERK pathways in cardiac differentiation of mouse embryonic stem cells.Molecular and cellular biochemistry · 2013Article
- Proteins and small molecules for cellular regenerative medicine.Physiological reviews · 2013Review
- Present state and future perspectives of using pluripotent stem cells in toxicology research.Archives of toxicology · 2011Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundEmbryonic stem (ES) cells differentiate into cardiac phenotypes representing early pacemaker-, atrial-, ventricular-, and sinus node-like cells, however, ES-derived specification into sinus nodal cells is not yet known. By using the naphthylamine derivative of urea, suramin, we were able to follow the process of cardiac specialization into sinus node-like cells.
methodsDifferentiating mouse ES cells were treated with suramin (500 µM) from day 5 to 7 of embryoid body formation, and cells were analysed for their differentiation potential via morphological analysis, flow cytometry, RT-PCR, immunohistochemistry and patch clamp analysis.
resultsApplication of suramin resulted in an increased number of cardiac cells, but inhibition of neuronal, skeletal muscle and definitive endoderm differentiation. Immediately after suramin treatment, a decreased mesendoderm differentiation was found. Brachyury, FGF10, Wnt8 and Wnt3a transcript levels were significantly down-regulated, followed by a decrease in mesoderm- and cardiac progenitor-specific markers BMP2, GATA4/5, Wnt11, Isl1, Nkx2.5 and Tbx5 immediately after removal of the substance. With continued differentiation, a significant up-regulation of Brachyury, FGF10 and GATA5 transcript levels was observed, whereas Nkx2.5, Isl1, Tbx5, BMP2 and Wnt11 levels were normalized to control levels. At advanced differentiation stages, sinus node-specific HCN4, Tbx2 and Tbx3 transcript levels were significantly up-regulated. Immunofluorescence and patch-clamp analysis confirmed the increased number of sinus node-like cells, and electrophysiological analysis revealed a lower number of atrial- and ventricular-like cardiomyocytes following suramin treatment.
conclusionWe conclude that the interference of suramin with the cardiac differentiation process modified mesoderm- and cardiac-specific gene expression resulting in enhanced formation of sinus node-like cells.
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