ArticleBiomaterials2009
Gene delivery via DNA incorporation within a biomimetic apatite coating.
Article in Biomaterials, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Nonviral Gene Delivery Embedded in Biomimetically Mineralized Matrices for Bone Tissue Engineering.Tissue engineering. Part A · 2021Article
- New Insights into the Therapeutic Applications of CRISPR/Cas9 Genome Editing in Breast Cancer.Genes · 2021Review
- Non-viral Gene Delivery Methods for Bone and Joints.Frontiers in bioengineering and biotechnology · 2020Review
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- Biomimetic Mineralization of Biomaterials Using Simulated Body Fluids for Bone Tissue Engineering and Regenerative Medicine<sup/>.Tissue engineering. Part A · 2017Review
- Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers.International journal of molecular sciences · 2015Article
- Functionalizing calcium phosphate biomaterials with antibacterial silver particles.Advanced materials (Deerfield Beach, Fla.) · 2013Article
- Biomimetic CaP coating incorporated with parathyroid hormone improves the osseointegration of titanium implant.Journal of materials science. Materials in medicine · 2012Article
- Exogenous mineralization of cell-seeded and unseeded collagen-chitosan hydrogels using modified culture medium.Acta biomaterialia · 2012Article
- Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer.Journal of materials science. Materials in medicine · 2012Article
- Using polymeric materials to control stem cell behavior for tissue regeneration.Birth defects research. Part C, Embryo today : reviews · 2012Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
Integrating inductivity with conductivity in a material may advance tissue engineering. An organic/inorganic hybrid was developed by incorporating plasmid DNA encoding for the beta-gal gene complexed with Lipofectamine 2000 (DNA-Lipoplex) within apatite via coprecipitation. It was hypothesized that this system will result in enhanced transfection efficiency compared to DNA-Lipoplexes adsorbed to the mineral surface and DNA coprecipitated without Lipofectamine 2000. PLGA films were cast onto glass slips and apatite and DNA were coprecipitated in modified simulated body fluid (mSBF). DNA-Lipoplex presence in mineral, DNA-Lipoplex stability (vs. coprecipitation time), and transfection efficiency (determined with C3H10T1/2 cells) as a function of coprecipitation time, DNA-Lipoplex concentration, and DNA incorporation method were studied. DNA-Lipoplex presence and spatial distribution on apatite were confirmed through fluorescence. Transfection efficiency was highest for 6h of DNA-Lipoplex coprecipitation. Differences in transfection efficiency were found between the DNA concentrations, with the highest efficiency for coprecipitation being 40 microg/ml (p < or = 0.009 relative to other coprecipitation concentrations). Significant differences in transfection efficiency existed between incorporation methods (p < 0.05) with the highest efficiency for DNA-Lipoplex coprecipitation. This hybrid material system not only integrates inductivity provided by the DNA and conductivity provided by the apatite, but it also has significant implications in non-viral gene delivery due to its ability to increase transfection efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.