ArticleMolecular microbiology2009
Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 84 citations in OpenAlex.
- EngineeringFrontiers in bioengineering and biotechnology · 2026Article
- Expression and characterization of a novel microbial GH9 glucanase, IDSGLUC9-4, isolated from sheep rumen.Animal bioscience · 2024Article
- Development of an efficient ClosTron system for gene disruption in Ruminiclostridium papyrosolvens.Applied microbiology and biotechnology · 2023Article
- Review
- Transcriptome Comparison between Two Strains ofJournal of fungi (Basel, Switzerland) · 2022Article
- Tuning of Gene Expression inACS synthetic biology · 2022Article
- Article
- ABC Transporters Required for Hexose Uptake by Clostridium phytofermentans.Journal of bacteriology · 2019Article
- Evolution of a Biomass-Fermenting Bacterium To Resist Lignin Phenolics.Applied and environmental microbiology · 2017Article
- Systems analysis in Cellvibrio japonicus resolves predicted redundancy of β-glucosidases and determines essential physiological functions.Molecular microbiology · 2017Article
- Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural.Applied and environmental microbiology · 2017Article
- Global repositioning of transcription start sites in a plant-fermenting bacterium.Nature communications · 2016Article
- Evolution of High Cellulolytic Activity in Symbiotic Streptomyces through Selection of Expanded Gene Content and Coordinated Gene Expression.PLoS biology · 2016Article
- Isolation of Genetically Tractable Most-Wanted Bacteria by Metaparental Mating.Scientific reports · 2015Article
- Physiology, Genomics, and Pathway Engineering of an Ethanol-Tolerant Strain of Clostridium phytofermentans.Applied and environmental microbiology · 2015Article
- Article
- Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum.Biotechnology for biofuels · 2015Article
- Genome and Transcriptome of Clostridium phytofermentans, Catalyst for the Direct Conversion of Plant Feedstocks to Fuels.PloS one · 2015Article
- Functional diversity of carbohydrate-active enzymes enabling a bacterium to ferment plant biomass.PLoS genetics · 2014Article
- Converting carbon dioxide to butyrate with an engineered strain of Clostridium ljungdahlii.mBio · 2014Article
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Summary Microbial cellulose degradation is a central part of the global carbon cycle and has great potential for the development of inexpensive, carbon-neutral biofuels from non-food crops. Clostridium phytofermentans has a repertoire of 108 putative glycoside hydrolases to break down cellulose and hemicellulose into sugars, which this organism then ferments primarily to ethanol. An understanding of cellulose degradation at the molecular level requires learning the different roles of these hydrolases. In this study, we show that interspecific conjugation with Escherichia coli can be used to transfer a plasmid into C. phytofermentans that has a resistance marker, an origin of replication that can be selectively lost, and a designed group II intron for efficient, targeted chromosomal insertions without selection. We applied these methods to disrupt the cphy3367 gene, which encodes the sole family 9 glycoside hydrolase (GH9) in the C. phytofermentans genome. The GH9-deficient strain grew normally on some carbon sources such as glucose, but had lost the ability to degrade cellulose. Although C. phytofermentans upregulates the expression of numerous enzymes to break down cellulose, this process thus relies upon a single, key hydrolase, Cphy3367.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.