ArticleJournal of medical virology2009
Development of a consensus microarray method for identification of some highly pathogenic viruses.
Article in Journal of medical virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites.Parasites & vectors · 2017Article
- A Novel Pan-BioMed research international · 2017Article
- DNA microarray for detection of gastrointestinal viruses.Journal of clinical microbiology · 2015Article
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- Evaluation of a field-portable DNA microarray platform and nucleic acid amplification strategies for the detection of arboviruses, arthropods, and bloodmeals.The American journal of tropical medicine and hygiene · 2013Article
- Multi-gene detection and identification of mosquito-borne RNA viruses using an oligonucleotide microarray.PLoS neglected tropical diseases · 2013Article
- Mosquito cellular factors and functions in mediating the infectious entry of chikungunya virus.PLoS neglected tropical diseases · 2013Article
- Metagenomics and the molecular identification of novel viruses.Veterinary journal (London, England : 1997) · 2011Review
- Molecular and nanotechnologic approaches to etiologic diagnosis of infectious syndromes.Molecular diagnosis & therapy · 2011Review
- A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses.Virology journal · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Some highly pathogenic viruses, such as Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Hanta virus, SARS-CoV, and H5N1 avian influenza virus can cause severe infectious diseases. However, the consensus method for detecting these viruses has not been well established. A rapid and sensitive microarray approach for detection of these viruses and a panel of specific probes covering nine genera and 16 virus species were designed. 70-mer oligonucleotides were used at the genus level and 50-mer oligonucleotides were at the species level, respectively. To decrease the interference of the host genome in hybridization, the consensus genus primers were designed and used to reverse transcribe only virus genome. The synthesis of the second strand was carried out with a random primer sequence (5'-GTTTCCCAGTAGGTCTCNNNNNNNN-3'). The amplified products were labeled and processed for microarray analyses. This microarray-based method used the highly conserved consensus primers to synthesize specifically the virus cDNA and could identify effectively Chikungunya virus, Japanese encephalitis virus, Yellow fever virus, Dengue virus, Tick borne encephalitis virus, and H5N1 avian influenza virus. Using this method, one unknown virus isolated from pig brain in Shanxi Province, China was identified. This method may have an important potential application for the diagnosis of virus infection.
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