ArticleNucleic acids research2009
A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 185 citations in OpenAlex.
- Polymers as Efficient Non-Viral Gene Delivery Vectors: The Role of the Chemical and Physical Architecture of Macromolecules.Polymers · 2024Review
- Effect of phosphorylation of protamine-like cationic peptide on the binding affinity to DNA.Biophysical journal · 2022Article
- Visualizing Disordered Single-Stranded RNA: Connecting Sequence, Structure, and Electrostatics.Journal of the American Chemical Society · 2020Article
- A Comprehensive Biophysical Analysis of the Effect of DNA Binding Drugs on Protamine-induced DNA Condensation.Scientific reports · 2019Article
- Competitive Binding of MgBiophysical journal · 2018Article
- Opposite effect of polyamines on In vitro gene expression: Enhancement at low concentrations but inhibition at high concentrations.PloS one · 2018Article
- Selective condensation of DNA by aminoglycoside antibiotics.European biophysics journal : EBJ · 2016Article
- Systematic Comparisons of Formulations of Linear Oligolysine Peptides with siRNA and Plasmid DNA.Chemical biology & drug design · 2016Article
- The structure and intermolecular forces of DNA condensates.Nucleic acids research · 2016Article
- Sensitivities to parameterization in the size-modified Poisson-Boltzmann equation.The Journal of chemical physics · 2014Article
- Why double-stranded RNA resists condensation.Nucleic acids research · 2014Article
- Ion competition in condensed DNA arrays in the attractive regime.Biophysical journal · 2013Article
- A comparison of DNA compaction by arginine and lysine peptides: a physical basis for arginine rich protamines.Biochemistry · 2013Article
- Article
- Salt effects on condensed protamine-DNA assemblies: anion binding and weakening of attraction.The journal of physical chemistry. B · 2011Article
- The effects of histone H4 tail acetylations on cation-induced chromatin folding and self-association.Nucleic acids research · 2011Article
- Electrostatic origin of salt-induced nucleosome array compaction.Biophysical journal · 2010Article
Corrections and comments
- Erratum issued
- Republished as
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a systematic study of the condensation of plasmid DNA by oligocations with variation of the charge, Z, from +3 to +31. The oligocations include a series of synthetic linear epsilon-oligo(l-lysines), (denoted epsilonKn, n = 3-10, 31; n is the number of lysines equal to the ligand charge) and branched alpha-substituted homologues of epsilonK10: epsilonYK10, epsilonLK10 (Z = +10); epsilonRK10, epsilonYRK10 and epsilonLYRK10 (Z = +20). Data were obtained by light scattering, UV absorption monitored precipitation assay and isothermal titration calorimetry in a wide range concentrations of DNA and monovalent salt (KCl, C(KCl)). The dependence of EC(50) (ligand concentration at the midpoint of DNA condensation) on C(KCl) shows the existence of a salt-independent regime at low C(KCl) and a salt-dependent regime with a steep rise of EC(50) with increase of C(KCl). Increase of the ligand charge shifts the transition from the salt-independent to salt-dependent regime to higher C(KCl). A novel and simple relationship describing the EC(50) dependence on DNA concentration, charge of the ligand and the salt-dependent dissociation constant of the ligand-DNA complex is derived. For the epsilon-oligolysines epsilonK3-epsilonK10, the experimental dependencies of EC(50) on C(KCl) and Z are well-described by an equation with a common set of parameters. Implications from our findings for understanding DNA condensation in chromatin are discussed.
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