ArticleNucleic acids research2009
Phage T4 mobE promotes trans homing of the defunct homing endonuclease I-TevIII.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity.Nature communications · 2026Article
- Neighboring inteins interfere with one another's homing capacity.PNAS nexus · 2023Article
- Diverse infective and lytic machineries identified in genome analysis of tailed coliphages against broad spectrum multidrug-resistant Escherichia coli.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Characterization of crAss-like phage isolates highlights Crassvirales genetic heterogeneity and worldwide distribution.Nature communications · 2023Article
- Genomic characterization of a new phage BUCT541 againstFrontiers in microbiology · 2022Article
- Systemic Expression, Purification, and Initial Structural Characterization of Bacteriophage T4 Proteins Without Known Structure Homologs.Frontiers in microbiology · 2021Article
- The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii.Virus genes · 2020Article
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease.Nucleic acids research · 2013Article
- Genome-wide characterization of Vibrio phage φpp2 with unique arrangements of the mob-like genes.BMC genomics · 2012Article
- The protein gp74 from the bacteriophage HK97 functions as a HNH endonuclease.Protein science : a publication of the Protein Society · 2012Article
- Learning to live together: mutualism between self-splicing introns and their hosts.BMC biology · 2011Review
- Genomes of the T4-related bacteriophages as windows on microbial genome evolution.Virology journal · 2010Review
- Mobile DNA elements in T4 and related phages.Virology journal · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Homing endonucleases are site-specific DNA endonucleases that typically function as mobile genetic elements by introducing a double-strand break (DSB) in genomes that lack the endonuclease, resulting in a unidirectional gene conversion event that mobilizes the homing endonuclease gene and flanking DNA. Here, we characterize phage T4-encoded mobE, a predicted free-standing HNH family homing endonuclease. We show that mobE is promoterless and dependent on upstream transcription for expression, and that an internal intrinsic terminator regulates mobE transcript levels. Crucially, in vivo mapping experiments revealed a MobE-dependent, strand-specific nick in the non-coding strand of the nrdB gene of phage T2. An internal deletion of the predicted HNH catalytic motif of MobE abolishes nicking, and reduces high-frequency inheritance of mobE. Sequence polymorphisms of progeny phage that inherit mobE are consistent with DSB repair pathways. Significantly, we found that mobility of the neighboring I-TevIII, a defunct homing endonuclease encoded within a group I intron interrupting the nrdB gene of phage T4, was dependent on an intact mobE gene. Thus, our data indicate that the stagnant nrdB intron and I-TevIII are mobilized in trans as a consequence of a MobE-dependent gene conversion event, facilitating persistence of genetic elements that have no inherent means of promoting their own mobility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.