ArticleJournal of the American Chemical Society2009
Microfluidic device for single-molecule experiments with enhanced photostability.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- An optofluidic antenna for enhancing the sensitivity of single-emitter measurements.Nature communications · 2024Article
- Trends in Single-Molecule Total Internal Reflection Fluorescence Imaging and Their Biological Applications with Lab-on-a-Chip Technology.Sensors (Basel, Switzerland) · 2023Review
- The biophysics of disordered proteins from the point of view of single-molecule fluorescence spectroscopy.Essays in biochemistry · 2022Article
- Integrating single-molecule spectroscopy and simulations for the study of intrinsically disordered proteins.Methods (San Diego, Calif.) · 2021Article
- Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.Chemphyschem : a European journal of chemical physics and physical chemistry · 2021Article
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Ratiometric Single-Molecule FRET Measurements to Probe Conformational Subpopulations of Intrinsically Disordered Proteins.Current protocols in chemical biology · 2020Article
- The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes.The Journal of cell biology · 2020Article
- Nanoscale devices for linkerless long-term single-molecule observation.Current opinion in biotechnology · 2016Review
- Deciphering Complexity in Molecular Biophysics with Single-Molecule Resolution.Journal of molecular biology · 2016Review
- A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization.Nature communications · 2016Article
- Continuous throughput and long-term observation of single-molecule FRET without immobilization.Nature methods · 2014Article
- Ultra-stable organic fluorophores for single-molecule research.Chemical Society reviews · 2014Review
- Shedding light on protein folding landscapes by single-molecule fluorescence.Chemical Society reviews · 2014Review
- Superhydrophobic photosensitizers. Mechanistic studies of (1)O2 generation in the plastron and solid/liquid droplet interface.Journal of the American Chemical Society · 2013Article
- Microfluidic mixer designed for performing single-molecule kinetics with confocal detection on timescales from milliseconds to minutes.Nature protocols · 2013Article
- Simple method to enhance the photostability of the fluorescence reporter R6G for prolonged single-molecule studies.The journal of physical chemistry. A · 2013Article
- Studies of bacterial aerotaxis in a microfluidic device.Lab on a chip · 2012Article
- Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.Molecular bioSystems · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.