ArticleJournal of RNAi and gene silencing : an international journal of RNA and gene targeting research2008
Identification and characterisation of the porcine 7SK RNA polymerase III promoter for short hairpin RNA expression.
Article in Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Conserved Herpesvirus Kinase ORF36 Activates B2 Retrotransposons during Murine Gammaherpesvirus Infection.Journal of virology · 2020Article
- Identification and comparison of the porcine H1, U6, and 7SK RNA polymerase III promoters for short hairpin RNA expression.Mammalian genome : official journal of the International Mammalian Genome Society · 2020Article
- Identification and validation of a Schistosoma japonicum U6 promoter.Parasites & vectors · 2017Article
- Identification and characterization of buffalo 7SK and U6 pol III promoters and application for expression of short hairpin RNAs.International journal of molecular sciences · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNA polymerase III (pol III) type III promoters U6 and 7SK are routinely used to express short hairpin RNA (shRNA) molecules from a DNA construct. In this study, we identified, characterised and compared the porcine 7SK promoter in porcine (homologous) and non-porcine (heterologous) derived cell lines. The porcine 7SK small nuclear RNA (snRNA) was identified by alignment with known sequences and further characterisation of the upstream regions determined the presence of typical RNA pol III sequence motifs. The porcine 7SK (po7SK) promoter was cloned and a one-step PCR strategy used to construct shRNA expression cassettes. The 7SK promoter activity was quantified by knockdown of the exogenous reporter gene encoding the enhanced green fluorescent protein (EGFP). Results indicated the po7SK promoter was functional in both homologous and heterologous cells lines. The identification and characterisation of the porcine RNA pol III promoter will contribute to the area of RNAi delivery and further develop our understanding of RNA promoter structure and function.
Indexed as
Identifiers
19771238PMC2737243W301534468What OpenQuestion holds
Registered trials
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