Evidence map›Paper›PMID 19771226›Full record

ArticleJournal of RNAi and gene silencing : an international journal of RNA and gene targeting research2006

3'-Poly(A) tail enhances siRNA activity against exogenous reporter genes in MCF-7 cells.

Jie Li, Guang Yang, Shaohua Li, Guojun Cao, Qiang Zhao, Xuemei Liu, Ming Fan, Beifen Shen, Ningsheng Shao

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Jie LiBeijing Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China.
Guang Yang
Shaohua Li
Guojun Cao
Qiang Zhao
Xuemei Liu
Ming Fan
Beifen Shen
Ningsheng Shao
Beijing Institute of Education · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA interference (RNAi) is a powerful tool in the study of gene function. We added poly(A) tails to the 3' ends of siRNA antisense strands by in vitro transcription, and investigated the silencing effects of poly (A)-tailed siRNAs on enhanced green fluorescence protein (EGFP) and red fluorescence protein (RFP) genes. The results of this study showed that siRNAs with single-stranded 3'-poly(A) tails at antisense strands had noticeably stronger silencing effect on exogenous reporter genes than their corresponding parental forms. The enhanced silencing effect appears to be related to the length of poly(A) but was non siRNA sequence-specific. Furthermore, our results demonstrate that weakly-activated PKR and reduced stability of mRNAs of exogenous reporter genes in vivo may be the possible mechanisms of this non-specific enhanced silencing effect. Our findings are likely to be of value in designing siRNAs with enhanced activity.

Indexed as

enhanced gene silencingnon-specificpoly(A) tailsiRNA

Identifiers

PMID19771226
PMCPMC2737218
OpenAlexW114240664

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.