ArticleJournal of RNAi and gene silencing : an international journal of RNA and gene targeting research2005
Frog Prince transposon-based RNAi vectors mediate efficient gene knockdown in human cells.
Article in Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- RNAi combining Sleeping Beauty transposon system inhibits ex vivo expression of foot-and-mouth disease virus VP1 in transgenic sheep cells.Scientific reports · 2017Article
- Primate-specific endogenous retrovirus-driven transcription defines naive-like stem cells.Nature · 2014Article
- Extracellular deposition of matrilin-2 controls the timing of the myogenic program during muscle regeneration.Journal of cell science · 2014Article
- Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons.Nature protocols · 2014Article
- Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells.PLoS genetics · 2014Article
- Characterization of human Spartan/C1orf124, an ubiquitin-PCNA interacting regulator of DNA damage tolerance.Nucleic acids research · 2012Article
- Article
- Triggered release of siRNA from poly(ethylene glycol)-protected, pH-dependent liposomes.Journal of controlled release : official journal of the Controlled Release Society · 2008Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a stable RNA interference (RNAi) delivery system that is based on the Frog Prince transposable element. This plasmid-based vector system combines the gene silencing capabilities of H1 polymerase III promoter-driven short hairpin RNAs (shRNA) with the advantages of stable and efficient genomic integration of the shRNA cassette mediated by transposition. We show that the Frog Prince-based shRNA expressing system can efficiently knock down the expression of both exogenous as well as endogenous genes in human cells. Furthermore, we use the Frog Prince-based system to study the effect of knockdown of the DNA repair factor Ku70 on transposition of the Sleeping Beauty transposon. Transposon-mediated genomic integration ensures that the shRNA expression cassette and a selectable marker gene within the transposon remain intact and physically linked. We demonstrate that a major advantage of our vector system over plasmid-based shRNA delivery is both its enhanced frequency of intact genomic integration as well as higher target suppression in transgenic human cells. Due to its simplicity and effectiveness, transposon-based RNAi is an emerging tool to facilitate analysis of gene function through the establishment of stable loss-of-function cell lines.
Indexed as
Identifiers
19771210PMC2737204What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.