ArticlePloS one2009
Estrogen/estrogen receptor alpha signaling in mouse posterofrontal cranial suture fusion.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 60 citations in OpenAlex.
- Age-dependent development of the interincisive suture: a CT-based study on prevalence and dimensions.Anatomical science international · 2026Article
- Integrative in-silico and in-vitro analysis of taurine and vitamin B12 in modulating PPARγ and Wnt signaling in hyperhomocysteinemia-induced osteoporosis.Biology direct · 2024Article
- Clomiphene Citrate and Enclomiphene Hydrochloride Exposure Is Associated With Interfrontal Suture Fusion in Zebrafish.Plastic surgery (Oakville, Ont.) · 2023Article
- Enhanced Osteoinductivity of Demineralized Bone Matrix with Noggin Suppression in Polymer Matrix.Advanced biology · 2021Article
- Loss of Angelman Syndrome Protein E6AP Disrupts a Novel Antagonistic Estrogen-Retinoic Acid Transcriptional Crosstalk in Neurons.Molecular neurobiology · 2018Article
- Homocysteine as a Pathological Biomarker for Bone Disease.Journal of cellular physiology · 2017Review
- Enhanced Mandibular Bone Repair by Combined Treatment of Bone Morphogenetic Protein 2 and Small-Molecule Phenamil.Tissue engineering. Part A · 2017Article
- In vivo expression and regulation of genes associated with vascularization during early response of sutures to tensile force.Journal of bone and mineral metabolism · 2017Article
- Magnetic Nanoparticle-Based Upregulation of B-Cell Lymphoma 2 Enhances Bone Regeneration.Stem cells translational medicine · 2017Article
- Calvarial Defect Healing Induced by Small Molecule Smoothened Agonist.Tissue engineering. Part A · 2016Article
- A Novel Method of Human Adipose-Derived Stem Cell Isolation with Resultant Increased Cell Yield.Plastic and reconstructive surgery · 2016Article
- Activation of the IGF1 pathway mediates changes in cellular contractility and motility in single-suture craniosynostosis.Journal of cell science · 2016Article
- Role of bone morphogenetic protein-2 in osteogenic differentiation of mesenchymal stem cells.Molecular medicine reports · 2015Article
- Article
- Delivery of Phenamil Enhances BMP-2-Induced Osteogenic Differentiation of Adipose-Derived Stem Cells and Bone Formation in Calvarial Defects.Tissue engineering. Part A · 2015Article
- Article
- Isolation and enrichment of human adipose-derived stromal cells for enhanced osteogenesis.Journal of visualized experiments : JoVE · 2015Article
- Osteogenic differentiation of human amniotic epithelial cells and its application in alveolar defect restoration.Stem cells translational medicine · 2014Article
- 10(-7) m 17β-oestradiol enhances odonto/osteogenic potency of human dental pulp stem cells by activation of the NF-κB pathway.Cell proliferation · 2013Article
- Human perivascular stem cells show enhanced osteogenesis and vasculogenesis with Nel-like molecule I protein.Tissue engineering. Part A · 2013Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundWhile premature suture fusion, or craniosynostosis, is a relatively common condition, the cause is often unknown. Estrogens are associated with growth plate fusion of endochondral bones. In the following study, we explore the previously unknown significance of estrogen/estrogen receptor signaling in cranial suture biology. METHODOLOGY/PRINCIPAL
findingsFirstly, estrogen receptor (ER) expression was examined in physiologically fusing (posterofrontal) and patent (sagittal) mouse cranial sutures by quantitative RT-PCR. Next, the cranial suture phenotype of ER alpha and ER beta knockout (alphaERKO, betaERKO) mice was studied. Subsequently, mouse suture-derived mesenchymal cells (SMCs) were isolated; the effects of 17-beta estradiol or the estrogen antagonist Fulvestrant on gene expression, osteogenic and chondrogenic differentiation were examined in vitro. Finally, in vivo experiments were performed in which Fulvestrant was administered subcutaneously to the mouse calvaria. Results showed that increased ERalpha but not ERbeta transcript abundance temporally coincided with posterofrontal suture fusion. The alphaERKO but not betaERKO mouse exhibited delayed posterofrontal suture fusion. In vitro, addition of 17-beta estradiol enhanced both osteogenic and chondrogenic differentiation in suture-derived mesenchymal cells, effects reversible by Fulvestrant. Finally, in vivo application of Fulvestrant significantly diminished calvarial osteogenesis, inhibiting suture fusion. CONCLUSIONS/SIGNIFICANCE: Estrogen signaling through ERalpha but not ERbeta is associated with and necessary for normal mouse posterofrontal suture fusion. In vitro studies suggest that estrogens may play a role in osteoblast and/or chondrocyte differentiation within the cranial suture complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.