ArticleActa crystallographica. Section D, Biological crystallography2009
Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity.
Article in Acta crystallographica. Section D, Biological crystallography, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 13 citations in OpenAlex.
- Structural insights into interdomain interactions inJournal of structural biology: X · 2026Article
- Sulfation pathways from red to green.The Journal of biological chemistry · 2019Review
- Characterization of Entamoeba histolytica adenosine 5'-phosphosulfate (APS) kinase; validation as a target and provision of leads for the development of new drugs against amoebiasis.PLoS neglected tropical diseases · 2019Article
- Recapitulating the Structural Evolution of Redox Regulation in Adenosine 5'-Phosphosulfate Kinase from Cyanobacteria to Plants.The Journal of biological chemistry · 2015Article
- Crystal structures of the kinase domain of the sulfate-activating complex in Mycobacterium tuberculosis.PloS one · 2015Article
- Adenosine-5'-phosphosulfate--a multifaceted modulator of bifunctional 3'-phospho-adenosine-5'-phosphosulfate synthases and related enzymes.The FEBS journal · 2013Review
- Redox-linked gating of nucleotide binding by the N-terminal domain of adenosine 5'-phosphosulfate kinase.The Journal of biological chemistry · 2013Article
- Nucleotide binding site communication in Arabidopsis thaliana adenosine 5'-phosphosulfate kinase.The Journal of biological chemistry · 2012Article
- Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Cloning, expression and bioinformatics analysis of ATP sulfurylase from Acidithiobacillus ferrooxidans ATCC 23270 in Escherichia coli.Bioinformation · 2012Article
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3 authors at 1 institution in 1 country.
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Abstract
The Tbd_0210 gene of the chemolithotrophic bacterium Thiobacillus denitrificans is annotated to encode a 60.5 kDa bifunctional enzyme with ATP sulfurylase and APS kinase activity. This putative bifunctional enzyme was cloned, expressed and structurally characterized. The 2.95 A resolution X-ray crystal structure reported here revealed a hexameric assembly with D(3) symmetry. Each subunit contains a large N-terminal sulfurylase-like domain and a C-terminal APS kinase domain reminiscent of the two-domain fungal ATP sulfurylases of Penicillium chrysogenum and Saccharomyces cerevisiae, which also exhibit a hexameric assembly. However, the T. denitrificans enzyme exhibits numerous structural and sequence differences in the N-terminal domain that render it inactive with respect to ATP sulfurylase activity. Surprisingly, the C-terminal domain does indeed display APS kinase activity, indicating that this gene product is a true APS kinase. Therefore, these results provide the first structural insights into a unique hexameric APS kinase that contains a nonfunctional ATP sulfurylase-like domain of unknown function.
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