ArticleJournal of molecular biology2009
The on-off switch in regulated myosins: different triggers but related mechanisms.
Article in Journal of molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Article
- Calcium-mediated regulation of recombinant hybrids of full-length Physarum myosin heavy chain with Physarum/scallop myosin light chains.Acta biochimica et biophysica Sinica · 2016Article
- Two Essential Light Chains Regulate the MyoA Lever Arm To Promote Toxoplasma Gliding Motility.mBio · 2015Article
- Sequential myosin phosphorylation activates tarantula thick filament via a disorder-order transition.Molecular bioSystems · 2015Article
- The apicomplexan glideosome and adhesins - Structures and function.Journal of structural biology · 2015Review
- Role of the essential light chain in the activation of smooth muscle myosin by regulatory light chain phosphorylation.Journal of structural biology · 2014Article
- Flexibility within the heads of muscle myosin-2 molecules.Journal of molecular biology · 2014Article
- Two structural motifs within canonical EF-hand calcium-binding domains identify five different classes of calcium buffers and sensors.PloS one · 2014Article
- Myosin light chains: Teaching old dogs new tricks.Bioarchitecture · 2014Review
- Structural basis of the relaxed state of a Ca2+-regulated myosin filament and its evolutionary implications.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Allosteric communication in Dictyostelium myosin II.Journal of muscle research and cell motility · 2012Article
- Modification of interface between regulatory and essential light chains hampers phosphorylation-dependent activation of smooth muscle myosin.The Journal of biological chemistry · 2012Article
- Phosphorylated smooth muscle heavy meromyosin shows an open conformation linked to activation.Journal of molecular biology · 2012Article
- A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments.Journal of molecular biology · 2011Article
- Crystal structure of a phosphorylated light chain domain of scallop smooth-muscle myosin.Biophysical journal · 2011Article
- Quantitative in vivo analyses reveal calcium-dependent phosphorylation sites and identifies a novel component of the Toxoplasma invasion motor complex.PLoS pathogens · 2011Article
- Broad disorder and the allosteric mechanism of myosin II regulation by phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Visualizing key hinges and a potential major source of compliance in the lever arm of myosin.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Essential "ankle" in the myosin lever arm.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In regulated myosin, motor and enzymatic activities are toggled between the on-state and off-state by a switch located on its lever arm domain, here called the regulatory domain (RD). This region consists of a long alpha-helical "heavy chain" stabilized by a "regulatory" light chain (RLC) and an "essential" light chain (ELC). The on-state is activated by phosphorylation of the RLC of vertebrate smooth muscle RD or by direct binding of Ca(2+) to the ELC of molluscan RD. Crystal structures are available only for the molluscan RD. To understand in more detail the pathway between the on-state and the off-state, we have now also determined the crystal structure of a molluscan (scallop) RD in the absence of Ca(2+). Our results indicate that loss of Ca(2+) abolishes most of the interactions between the light chains and may increase the flexibility of the RD heavy chain. We propose that disruption of critical links with the C-lobe of the RLC is the key event initiating the off-state in both smooth muscle myosins and molluscan myosins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.