ArticleJournal of proteome research2009
Differential secreted proteome approach in murine model for candidate biomarker discovery in colon cancer.
Article in Journal of proteome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Glyoxal-induced disruption of tumor cell progression in breast cancer.Molecular and clinical oncology · 2023Article
- Targeted Treatment of Colon Cancer with Aptamer-Guided Albumin Nanoparticles Loaded with Docetaxel.International journal of nanomedicine · 2020Article
- Targeted Therapy of Colon Cancer by Aptamer-Guided Holliday Junctions Loaded with Doxorubicin.International journal of nanomedicine · 2020Article
- Quantification of Serum High Mobility Group Box 1 by Liquid Chromatography/High-Resolution Mass Spectrometry: Implications for Its Role in Immunity, Inflammation, and Cancer.Analytical chemistry · 2018Article
- Proteins are potent biomarkers to detect colon cancer progression.Saudi journal of biological sciences · 2017Article
- A Timely Shift from Shotgun to Targeted Proteomics and How It Can Be Groundbreaking for Cancer Research.Frontiers in oncology · 2017Review
- Serum apolipoprotein A-1 quantification by LC-MS with a SILAC internal standard reveals reduced levels in smokers.Bioanalysis · 2015Article
- Review
- Human platelets as a platform to monitor metabolic biomarkers using stable isotopes and LC-MS.Bioanalysis · 2013Article
- SILAC-based quantitative proteomic analysis of gastric cancer secretome.Proteomics. Clinical applications · 2013Article
- A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.PloS one · 2013Article
- Sample prefractionation for mass spectrometry quantification of low-abundance membrane proteins.Analytical chemistry · 2012Article
- Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions.Nature methods · 2012Review
- Lectin chromatography/mass spectrometry discovery workflow identifies putative biomarkers of aggressive breast cancers.Journal of proteome research · 2012Article
- Relative quantification of serum proteins from pancreatic ductal adenocarcinoma patients by stable isotope dilution liquid chromatography-mass spectrometry.Journal of proteome research · 2012Article
- SILEC: a protocol for generating and using isotopically labeled coenzyme A mass spectrometry standards.Nature protocols · 2011Article
- Stable-isotope dilution LC–MS for quantitative biomarker analysis.Bioanalysis · 2010Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The complexity and heterogeneity of the plasma proteome have presented significant challenges in the identification of protein changes associated with tumor development. We used cell culture as a model system and identified differentially expressed, secreted proteins which may constitute serological biomarkers. A stable isotope labeling by amino acids in cell culture (SILAC) approach was used to label the entire secreted proteomes of the CT26 murine colon cancer cell line and normal young adult mouse colon (YAMC) cell line, thereby creating a stable isotope labeled proteome (SILAP) standard. This SILAP standard was added to unlabeled murine CT26 colon cancer cell or normal murine YAMC colon epithelial cell secreted proteome samples. A multidimensional approach combining isoelectric focusing (IEF), strong cation exchange (SCX) followed by reversed phase liquid chromatography was used for extensive protein and peptide separation. A total of 614 and 929 proteins were identified from the YAMC and CT26 cell lines, with 418 proteins common to both cell lines. Twenty highly abundant differentially expressed proteins from these groups were selected for liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS) analysis in sera. Differential secretion into the serum was observed for several proteins when Apc(min) mice were compared with control mice. These findings were then confirmed by Western blot analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.