ArticleBiochemistry2009
Molecular mechanism for lateral lipid diffusion between the outer membrane external leaflet and a beta-barrel hydrocarbon ruler.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Phospholipid composition strongly affects the assembly of β barrel proteins into purified bacterial outer membranes.Nature communications · 2026Article
- A computational model for lipid-anchored polysaccharide export by the outer membrane protein GfcD.Biophysical journal · 2024Article
- Ceramides: Nutrient Signals that Drive Hepatosteatosis.Journal of lipid and atherosclerosis · 2020Review
- Targeting a ceramide double bond improves insulin resistance and hepatic steatosis.Science (New York, N.Y.) · 2019Article
- Influence of Protein Scaffold on Side-Chain Transfer Free Energies.Biophysical journal · 2017Article
- Insertion of proteins and lipopolysaccharide into the bacterial outer membrane.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens.Structure (London, England : 1993) · 2016Article
- Lipopolysaccharide transport and assembly at the outer membrane: the PEZ model.Nature reviews. Microbiology · 2016Review
- Lipopolysaccharide transport to the cell surface: periplasmic transport and assembly into the outer membrane.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane.Scientific reports · 2015Article
- Lipopolysaccharide is inserted into the outer membrane through an intramembrane hole, a lumen gate, and the lateral opening of LptD.Structure (London, England : 1993) · 2015Article
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- PhoPQ regulates acidic glycerophospholipid content of the Salmonella Typhimurium outer membrane.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Wzi is an outer membrane lectin that underpins group 1 capsule assembly in Escherichia coli.Structure (London, England : 1993) · 2013Article
- Inscribing the perimeter of the PagP hydrocarbon ruler by site-specific chemical alkylation.Biochemistry · 2010Article
- PagP crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler.Structure (London, England : 1993) · 2010Article
- A thiolate anion buried within the hydrocarbon ruler perturbs PagP lipid acyl chain selection.Biochemistry · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Membrane-intrinsic enzymes are embedded in lipids, yet how such enzymes interrogate lipid substrates remains a largely unexplored fundamental question. The outer membrane phospholipid:lipid A palmitoyltransferase PagP combats host immune defenses during infection and selects a palmitate chain using its beta-barrel interior hydrocarbon ruler. Both a molecular embrasure and crenel in Escherichia coli PagP display weakened transmembrane beta-strand hydrogen bonding to provide potential lateral routes for diffusion of the palmitoyl group between the hydrocarbon ruler and outer membrane external leaflet. Prolines in strands A and B lie beneath the dynamic L1 surface loop flanking the embrasure, whereas the crenel is flanked by prolines in strands F and G. Reversibly barricading the embrasure prevents lipid A palmitoylation without affecting the slower phospholipase reaction. Lys42Ala PagP is also a dedicated phospholipase, implicating this disordered L1 loop residue in lipid A recognition. The embrasure barricade additionally prevents palmitoylation of nonspecific fatty alcohols, but not miscible alcohols. Irreversibly barricading the crenel inhibits both lipid A palmitoylation and phospholipase reactions without compromising PagP structure. These findings indicate lateral palmitoyl group diffusion within the PagP hydrocarbon ruler is likely gated during phospholipid entry via the crenel and during lipid A egress via the embrasure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.