ArticleJournal of biomedical materials research. Part A2010
Influence of FGF2 and PEG hydrogel matrix properties on hMSC viability and spreading.
Article in Journal of biomedical materials research. Part A, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Applications of Biomaterials in 3D Cell Culture and Contributions of 3D Cell Culture to Drug Development and Basic Biomedical Research.International journal of molecular sciences · 2021Review
- Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers.Marine drugs · 2020Article
- Dual-delivery of FGF-2/CTGF from Silk Fibroin/PLCL-PEO Coaxial Fibers Enhances MSC Proliferation and Fibrogenesis.Scientific reports · 2017Article
- Combinatorial Method/High Throughput Strategies for Hydrogel Optimization in Tissue Engineering Applications.Gels (Basel, Switzerland) · 2016Review
- Hydrogel arrays formed via differential wettability patterning enable combinatorial screening of stem cell behavior.Acta biomaterialia · 2016Article
- Effects of low dose FGF-2 and BMP-2 on healing of calvarial defects in old mice.Experimental gerontology · 2015Article
- Differential effects of cell adhesion, modulus and VEGFR-2 inhibition on capillary network formation in synthetic hydrogel arrays.Biomaterials · 2014Article
- The influence of matrix degradation and functionality on cell survival and morphogenesis in PEG-based hydrogels.Macromolecular bioscience · 2013Article
- Growth factor delivery: how surface interactions modulate release in vitro and in vivo.Advanced drug delivery reviews · 2012Review
- Moving from static to dynamic complexity in hydrogel design.Nature communications · 2012Article
- Magnetic manipulation and spatial patterning of multi-cellular stem cell aggregates.Integrative biology : quantitative biosciences from nano to macro · 2011Article
- PEG hydrogels formed by thiol-ene photo-click chemistry and their effect on the formation and recovery of insulin-secreting cell spheroids.Biomaterials · 2011Article
- Biomimetic approaches to control soluble concentration gradients in biomaterials.Macromolecular bioscience · 2011Review
- "Humanized" stem cell culture techniques: the animal serum controversy.Stem cells international · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In this study, three-dimensional (3-D) poly(ethylene glycol) (PEG) hydrogel arrays were used to screen for the effects of fibroblast growth factor-2 (FGF2), combined with multiple hydrogel matrix parameters, on human mesenchymal stem cell (hMSC) viability and spreading. In particular, we examined the effects of FGF2 while co-varying hydrogel matrix degradability, cell adhesion ligand type, and cell adhesion ligand density. FGF2 significantly improved viability of hMSCs in a dose-dependent manner in both nondegrading and degrading PEG hydrogels in the absence of extracellular matrix-derived cell adhesion ligands. The presence of a small molecule that inhibits autophosphorylation of the FGF2 receptor blocked the effects of FGF2 on hMSC viability in PEG hydrogels, both in the presence and absence of the Arg-Gly-Asp-Ser-Pro (RGDSP) ligand. FGF2 effects on hMSC viability were less pronounced when FGF2 was presented in combination with the RGDSP cell adhesion ligand or the IKVAV cell adhesion ligand in nondegrading PEG hydrogels. Importantly, spread hMSC morphologies were observed and quantified in a select subset of hydrogel networks, which were degradable and included both FGF2 and RGDSP. These results indicate that the hydrogel arrays described here can be used to efficiently study the influence of soluble and insoluble hydrogel matrix parameters on stem cell behavior, and to identify synthetic, 3-D environments that promote specific hMSC behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.