ArticlePloS one2009
Restriction of HIV-1 replication in monocytes is abolished by Vpx of SIVsmmPBj.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- HIV-2 Vpx neutralizes host restriction factor SAMHD1 to promote viral pathogenesis.Scientific reports · 2021Article
- Alarmin S100A9 restricts retroviral infection by limiting reverse transcription in human dendritic cells.The EMBO journal · 2021Article
- Interference with SAMHD1 Restores Late Gene Expression of Modified Vaccinia Virus Ankara in Human Dendritic Cells and Abrogates Type I Interferon Expression.Journal of virology · 2019Article
- Tropism, intracerebral distribution, and transduction efficiency of HIV- and SIV-based lentiviral vectors after injection into the mouse brain: a qualitative and quantitative in vivo study.Histochemistry and cell biology · 2017Article
- TLR7/8 agonist induces a post-entry SAMHD1-independent block to HIV-1 infection of monocytes.Retrovirology · 2016Article
- Vpx rescue of HIV-1 from the antiviral state in mature dendritic cells is independent of the intracellular deoxynucleotide concentration.Retrovirology · 2014Article
- Antagonism of SAMHD1 is actively maintained in natural infections of simian immunodeficiency virus.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cellular and Biochemical Mechanisms of the Retroviral Restriction Factor SAMHD1.ISRN biochemistry · 2013Article
- SAMHD1 restricts HIV-1 infection in dendritic cells (DCs) by dNTP depletion, but its expression in DCs and primary CD4+ T-lymphocytes cannot be upregulated by interferons.Retrovirology · 2012Article
- Restricting HIV the SAMHD1 way: through nucleotide starvation.Nature reviews. Microbiology · 2012Article
- Neuropeptide receptor ligands as drugs for psychiatric diseases: the end of the beginning?Nature reviews. Drug discovery · 2012Review
- Measles virus glycoprotein-pseudotyped lentiviral vectors are highly superior to vesicular stomatitis virus G pseudotypes for genetic modification of monocyte-derived dendritic cells.Journal of virology · 2012Article
- SAMHD1-deficient CD14+ cells from individuals with Aicardi-Goutières syndrome are highly susceptible to HIV-1 infection.PLoS pathogens · 2011Article
- Primate and feline lentiviruses in current intrinsic immunity research: the cat is back.Veterinary immunology and immunopathology · 2011Review
- Comparative analysis of transduced primary human dendritic cells generated by the use of three different lentiviral vector systems.Molecular biotechnology · 2011Article
- Interaction of Vpx and apolipoprotein B mRNA-editing catalytic polypeptide 3 family member A (APOBEC3A) correlates with efficient lentivirus infection of monocytes.The Journal of biological chemistry · 2010Article
- Limelight on two HIV/SIV accessory proteins in macrophage infection: is Vpx overshadowing Vpr?Retrovirology · 2010Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman primary monocytes are refractory to infection with the human immunodeficiency virus 1 (HIV-1) or transduction with HIV-1-derived vectors. In contrast, efficient single round transduction of monocytes is mediated by vectors derived from simian immunodeficiency virus of sooty mangabeys (SIVsmmPBj), depending on the presence of the viral accessory protein Vpx. METHODS AND
findingsHere we analyzed whether Vpx of SIVsmmPBj is sufficient for transduction of primary monocytes by HIV-1-derived vectors. To enable incorporation of PBj Vpx into HIV-1 vector particles, a HA-Vpr/Vpx fusion protein was generated. Supplementation of HIV-1 vector particles with this fusion protein was not sufficient to facilitate transduction of human monocytes. However, monocyte transduction with HIV-1-derived vectors was significantly enhanced after delivery of Vpx proteins by virus-like particles (VLPs) derived from SIVsmmPBj. Moreover, pre-incubation with Vpx-containing VLPs restored replication capacity of infectious HIV-1 in human monocytes. In monocytes of non-human primates, single-round transduction with HIV-1 vectors was enabled.
conclusionVpx enhances transduction of primary human and even non-human monocytes with HIV-1-derived vectors, only if delivered in the background of SIVsmmPBj-derived virus-like particles. Thus, for accurate Vpx function the presence of SIVsmmPBj capsid proteins might be required. Vpx is essential to overcome a block of early infection steps in primary monocytes.
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