ArticleNature methods2009
Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms.
Article in Nature methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 625 papers.
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Who cites it
625 citing papers in PubMed, 983 citations in OpenAlex.
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- Systematic mapping of bacteriophage gene essentiality with HIDEN-SEQ.Nature microbiology · 2026Article
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- Genome microbiology at 20 years: who we are and where we are going.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Applications of transposon-insertion sequencing for understanding bacterial physiology.Microbial genomics · 2026Review
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- Coordinated RNA- and protein-templated synthesis of double-stranded DNA by a dual reverse transcriptase immune system.bioRxiv : the preprint server for biology · 2026Article
- Epistasis and co-adaptation in bacterial genome evolution.Nature reviews. Genetics · 2026Review
- GeneScanner: profiling genetic variation across bacterial populations.Microbial genomics · 2026Article
- Transposon mutant library reveals the complex regulatory network of biofilm formation inBiofilm · 2026Article
- CUPID-seq enables highly multiplexed amplicon sequencing via combinatorial in-line dual indexing.bioRxiv : the preprint server for biology · 2026Article
- Oligonucleotide genetics forbioRxiv : the preprint server for biology · 2026Article
- Unravelling the unique essential genes ofMicrobial genomics · 2026Article
- Article
565 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Biological pathways are structured in complex networks of interacting genes. Solving the architecture of such networks may provide valuable information, such as how microorganisms cause disease. Here we present a method (Tn-seq) for accurately determining quantitative genetic interactions on a genome-wide scale in microorganisms. Tn-seq is based on the assembly of a saturated Mariner transposon insertion library. After library selection, changes in frequency of each insertion mutant are determined by sequencing the flanking regions en masse. These changes are used to calculate each mutant's fitness. Using this approach, we determined fitness for each gene of Streptococcus pneumoniae, a causative agent of pneumonia and meningitis. A genome-wide screen for genetic interactions of five query genes identified both alleviating and aggravating interactions that could be divided into seven distinct categories. Owing to the wide activity of the Mariner transposon, Tn-seq has the potential to contribute to the exploration of complex pathways across many different species.
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