ArticleNature chemical biology2009
The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo.
Article in Nature chemical biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- 3D confinement physically regulates cell cycle progression in budding yeast.Nature communications · 2026Article
- Gravity as a Boundary Condition for the Evolution of Three-Dimensional Multicellularity.Life (Basel, Switzerland) · 2026Article
- Molecular and Structural Characterization of Foam Proteins from Mahanarva spectabilis (Distant, 1909) (Hemiptera: Cercopidae) Nymphs Reveals Adaptive Features and Potential Targets for Pest Control.Archives of insect biochemistry and physiology · 2026Article
- Functions of the Three Common Fungal Extracellular Membrane (CFEM) Domain-Containing Genes ofMicroorganisms · 2025Article
- Article
- Distinct TORC1 signalling branches regulate Adc17 proteasome assembly chaperone expression.Journal of cell science · 2024Article
- Activation of CWI pathway through high hydrostatic pressure, enhancing glycerol efflux via the aquaglyceroporin Fps1 inMolecular biology of the cell · 2023Article
- Arabidopsis root responses to salinity depend on pectin modification and cell wall sensing.Development (Cambridge, England) · 2022Article
- Structure of the Yeast Cell Wall Integrity Sensor Wsc1 Reveals an Essential Role of Surface-Exposed Aromatic Clusters.Journal of fungi (Basel, Switzerland) · 2022Article
- Regulation of Peroxisome Homeostasis by Post-Translational Modification in the Methylotrophic YeastFrontiers in cell and developmental biology · 2022Review
- Differential Requirement for the Cell Wall Integrity Sensor Wsc1p in Diploids Versus Haploids.Journal of fungi (Basel, Switzerland) · 2021Article
- Studying structure and functions of cell membranes by single molecule biophysical techniques.Biophysics reports · 2021Article
- Fungal Cell Wall Proteins and Signaling Pathways Form a Cytoprotective Network to Combat Stresses.Journal of fungi (Basel, Switzerland) · 2021Review
- With an Ear Up against the Wall: An Update on Mechanoperception inPlants (Basel, Switzerland) · 2021Review
- A Three-Dimensional Model of the Yeast Transmembrane Sensor Wsc1 Obtained by SMA-Based Detergent-Free Purification and Transmission Electron Microscopy.Journal of fungi (Basel, Switzerland) · 2021Article
- Article
- Plant cell wall integrity maintenance in model plants and crop species-relevant cell wall components and underlying guiding principles.Cellular and molecular life sciences : CMLS · 2020Review
- Article
- The Role of Mechanoperception in Plant Cell Wall Integrity Maintenance.Plants (Basel, Switzerland) · 2020Review
- Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high COBiotechnology and bioengineering · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report on in vivo measurement of the mechanical behavior of a cell surface sensor using single-molecule atomic force microscopy. We focus on the yeast wall stress component sensor Wsc1, a plasma membrane protein that is thought to function as a rigid probe of the cell wall status. We first map the distribution of individual histidine-tagged sensors on living yeast cells by scanning the cell surface with atomic force microscopy tips carrying nitrilotriacetate groups. We then show that Wsc1 behaves like a linear nanospring that is capable of resisting high mechanical force and of responding to cell surface stress. Both a genomic pmt4 deletion and the insertion of a stretch of glycines in Wsc1 result in substantial alterations in protein spring properties, supporting the important role of glycosylation at the extracellular serine/threonine-rich region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.