ArticleNucleic acids research2009
A SRS2 homolog from Arabidopsis thaliana disrupts recombinogenic DNA intermediates and facilitates single strand annealing.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- SRS2 is required for MUS81-dependent CO formation in zmm mutants.PLoS genetics · 2025Article
- The 2 faces of plant SUMOylation against viruses.PLoS pathogens · 2024Article
- Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators.Plant reproduction · 2023Review
- Downregulation of BarleyFrontiers in plant science · 2021Article
- Review
- Antagonism between BRCA2 and FIGL1 regulates homologous recombination.Nucleic acids research · 2019Article
- Geminivirus Replication Protein Impairs SUMO Conjugation of Proliferating Cellular Nuclear Antigen at Two Acceptor Sites.Journal of virology · 2018Article
- Protecting DNA from errors and damage: an overview of DNA repair mechanisms in plants compared to mammals.Cellular and molecular life sciences : CMLS · 2017Review
- The Arabidopsis thaliana homolog of the helicase RTEL1 plays multiple roles in preserving genome stability.The Plant cell · 2014Article
- RAD51B plays an essential role during somatic and meiotic recombination in Physcomitrella.Nucleic acids research · 2014Article
- Heteroduplex DNA position defines the roles of the Sgs1, Srs2, and Mph1 helicases in promoting distinct recombination outcomes.PLoS genetics · 2013Article
- A failure of meiotic chromosome segregation in a fbh1Delta mutant correlates with persistent Rad51-DNA associations.Nucleic acids research · 2011Article
- Rad51 inhibits translocation formation by non-conservative homologous recombination in Saccharomyces cerevisiae.PloS one · 2010Article
- Cdk1 targets Srs2 to complete synthesis-dependent strand annealing and to promote recombinational repair.PLoS genetics · 2010Article
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6 authors.
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Abstract
Genetic and biochemical analyses of SRS2 homologs in fungi indicate a function in the processing of homologous recombination (HR) intermediates. To date, no SRS2 homologs have been described and analyzed in higher eukaryotes. Here, we report the first biochemical characterization of an SRS2 homolog from a multicellular eukaryote, the plant Arabidopsis thaliana. We studied the basic properties of AtSRS2 and were able to show that it is a functional 3'- to 5'-helicase. Furthermore, we characterized its biochemical function on recombinogenic intermediates and were able to show the unwinding of nicked Holliday junctions (HJs) and partial HJs (PX junctions). For the first time, we demonstrated strand annealing activity for an SRS2 homolog and characterized its strand pairing activity in detail. Our results indicate that AtSRS2 has properties that enable it to be involved in different steps during the processing of recombination intermediates. On the one hand, it could be involved in the unwinding of an elongating invading strand from a donor strand, while on the other hand, it could be involved in the annealing of the elongated strand at a later step.
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