ArticleNucleic acids research2009
Tertiary network in mammalian mitochondrial tRNAAsp revealed by solution probing and phylogeny.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Guanosine 10 methylation by TRMT11-TRMT112 optimizes human mitochondrial tRNAs for efficient translation.Nucleic acids research · 2026Article
- In vivo structure profiling reveals human cytosolic and mitochondrial tRNA structurome and interactome in response to stress.Nature communications · 2025Article
- Molecular basis of human nuclear and mitochondrial tRNA 3' processing.Nature structural & molecular biology · 2025Article
- RNA modifying enzymes shape tRNA biogenesis and function.The Journal of biological chemistry · 2024Review
- Human transfer RNA modopathies: diseases caused by aberrations in transfer RNA modifications.The FEBS journal · 2021Review
- Relaxed sequence constraints favor mutational freedom in idiosyncratic metazoan mitochondrial tRNAs.Nature communications · 2020Article
- Naturally Occurring tRNAs With Non-canonical Structures.Frontiers in microbiology · 2020Review
- tRNA-Derived Small RNAs and Their Potential Roles in Cardiac Hypertrophy.Frontiers in pharmacology · 2020Review
- Mammalian NSUN2 introduces 5-methylcytidines into mitochondrial tRNAs.Nucleic acids research · 2019Article
- tRNA Modifications: Impact on Structure and Thermal Adaptation.Biomolecules · 2017Review
- A deafness-associated tRNAAsp mutation alters the m1G37 modification, aminoacylation and stability of tRNAAsp and mitochondrial function.Nucleic acids research · 2016Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs.Nucleic acids research · 2014Article
- Thermodynamic properties distinguish human mitochondrial aspartyl-tRNA synthetase from bacterial homolog with same 3D architecture.Nucleic acids research · 2013Article
- Kinetics of tRNA folding monitored by aminoacylation.RNA (New York, N.Y.) · 2012Article
- A compendium of human mitochondrial gene expression machinery with links to disease.Environmental and molecular mutagenesis · 2010Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary and secondary structures of mammalian mitochondrial (mt) tRNAs are divergent from canonical tRNA structures due to highly skewed nucleotide content and large size variability of D- and T-loops. The nonconservation of nucleotides involved in the expected network of tertiary interactions calls into question the rules governing a functional L-shaped three-dimensional (3D) structure. Here, we report the solution structure of human mt-tRNA(Asp) in its native post-transcriptionally modified form and as an in vitro transcript. Probing performed with nuclease S1, ribonuclease V1, dimethylsulfate, diethylpyrocarbonate and lead, revealed several secondary structures for the in vitro transcribed mt-tRNA(Asp) including predominantly the cloverleaf. On the contrary, the native tRNA(Asp) folds into a single cloverleaf structure, highlighting the contribution of the four newly identified post-transcriptional modifications to correct folding. Reactivities of nucleotides and phosphodiester bonds in the native tRNA favor existence of a full set of six classical tertiary interactions between the D-domain and the variable region, forming the core of the 3D structure. Reactivities of D- and T-loop nucleotides support an absence of interactions between these domains. According to multiple sequence alignments and search for conservation of Leontis-Westhof interactions, the tertiary network core building rules apply to all tRNA(Asp) from mammalian mitochondria.
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