ArticleThe Journal of molecular diagnostics : JMD2009
Effects of ozone exposure during microarray posthybridization washes and scanning.
Article in The Journal of molecular diagnostics : JMD, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Custom Array Comparative Genomic Hybridization: the Importance of DNA Quality, an Expert Eye, and Variant Validation.International journal of molecular sciences · 2017Article
- ELISA in the multiplex era: potentials and pitfalls.Proteomics. Clinical applications · 2015Review
- miRNA profiles of monocyte-lineage cells are consistent with complicated roles in HIV-1 restriction.Viruses · 2012Article
- Tourette syndrome: gene expression as a tool to discover drug targets.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2010Review
- Gene expression profiling of mouse embryos with microarrays.Methods in enzymology · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increasing prevalence of array-based comparative genomic hybridization in the clinical laboratory necessitates the implementation of quality control measures to attain accurate results with a high level of confidence. Environmental ozone is present in all industrialized cities and has been found to be detrimental to array data even at levels considered acceptable by US Environmental Protection Agency standards. In this study, we characterized the effect of ozone on microarray data on three different labeling platforms that use different fluorescent dyes (Cy3 and Cy5, Alexa Fluor 555 and Alexa Fluor 647, and Alexa Fluor 3 and Alexa Fluor 5) that are commonly used in array-based comparative genomic hybridization. We investigated the effects of ozone on microarray data by washing the array in variable ozone environments. In addition, we observed the effects of prolonged exposure to ozone on the microarray after washing in an ozone-free environment. Our results demonstrate the necessity of minimizing ozone exposure when washing and drying the microarray. We also found that washed microarrays produce the best results when immediately scanned; however, if a low-ozone environment is maintained, there will be little compromise in the data collected.
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Registered trials
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