ArticleApplied and environmental microbiology2009
Quadruplex real-time PCR assay for detection and identification of Vibrio cholerae O1 and O139 strains and determination of their toxigenic potential.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Evaluation of the Expression of Virulence Factors ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2025Article
- Genomic characterization ofFrontiers in microbiology · 2025Article
- Outbreak ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Pathways of exposure to Vibrio Cholerae in an urban informal settlement in Nairobi, Kenya.PLOS global public health · 2024Article
- Characterization of a Nonagglutinating Toxigenic Vibrio cholerae Isolate.Microbiology spectrum · 2023Article
- Review of Rapid Diagnostic Methods forThe East African health research journal · 2023Article
- Absolute quantification of viableFrontiers in microbiology · 2023Article
- Assay for Evaluating the Abundance ofPathogens (Basel, Switzerland) · 2022Article
- Development and Application of a Multiplex Fluorescent PCR for Shigella Detection and Species Identification.Journal of fluorescence · 2022Article
- Development and Validation of a Novel Real-time Assay for the Detection and Quantification ofFrontiers in public health · 2017Article
- Quantitative PCR and Digital PCR for Detection ofBioMed research international · 2017Article
- Quantitative detection of Vibrio cholera toxin by real-time and dynamic cytotoxicity monitoring.Journal of clinical microbiology · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio cholerae is a natural inhabitant of the aquatic environment. However, its toxigenic strains can cause potentially life-threatening diarrhea. A quadruplex real-time PCR assay targeting four genes, the cholera toxin gene (ctxA), the hemolysin gene (hlyA), O1-specific rfb, and O139-specific rfb, was developed for detection and differentiation of O1, O139, and non-O1, non-O139 strains and for prediction of their toxigenic potential. The specificity of the assay was 100% when tested against 70 strains of V. cholerae and 31 strains of non-V. cholerae organisms. The analytical sensitivity for detection of toxigenic V. cholerae O1 and O139 was 2 CFU per reaction with cells from pure culture. When the assay was tested with inoculated water from bullfrog feeding ponds, 10 CFU/ml could reliably be detected after culture for 3 h. The assay was more sensitive than the immunochromatographic assay and culture method when tested against 89 bullfrog samples and 68 water samples from bullfrog feeding ponds. The applicability of this assay was confirmed in a case study involving 15 bullfrog samples, from which two mixtures of nontoxigenic O1 and toxigenic non-O1/non-O139 strains were detected and differentiated. These data indicate that the quadruplex real-time PCR assay can both rapidly and accurately detect/identify V. cholerae and reliably predict the toxigenic potential of strains detected.
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