ArticleAmerican journal of respiratory cell and molecular biology2010
Directed expression of Cre in alveolar epithelial type 1 cells.
Article in American journal of respiratory cell and molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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53 citing papers in PubMed, 68 citations in OpenAlex.
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- Cdc42 regulates apical membrane fusion via the Rab11a-VAMP2 pathway in salivary gland acinar cells.bioRxiv : the preprint server for biology · 2025Article
- Magnetoactive,Cellular and molecular bioengineering · 2024Article
- Generation of human alveolar epithelial type I cells from pluripotent stem cells.Cell stem cell · 2024Article
- FGFR2 is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation.Nature communications · 2023Article
- FGFR2b is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation.Research square · 2023Article
- Lung inflammation and interstitial fibrosis by targeted alveolar epithelial type I cell death.Frontiers in immunology · 2023Article
- NLRP6 controls pulmonary inflammation from cigarette smoke in a gut microbiota-dependent manner.Frontiers in immunology · 2023Article
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- Activation of the Ae4 (Slc4a9) cation-driven ClAmerican journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Epithelial Stem and Progenitor Cells in Lung Repair and Regeneration.Annual review of physiology · 2021Review
- The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3.Nature communications · 2021Article
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- Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung.Developmental cell · 2020Article
- Cdc42 controls secretory granules morphology in rodent salivary glands in vivo.Communicative & integrative biology · 2020Article
- Interleukin-22 (IL-22) Binding Protein Constrains IL-22 Activity, Host Defense, and Oxidative Phosphorylation Genes during Pneumococcal Pneumonia.Infection and immunity · 2019Article
- Transcriptional control of lung alveolar type 1 cell development and maintenance by NK homeobox 2-1.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Pulmonary alveolar epithelium is comprised of two morphologically and functionally distinct cell types, alveolar epithelial type (AT) I and AT2 cells. Genetically modified mice with cell-specific Cre/loxP-mediated knockouts of relevant genes in each respective cell type would be useful to help elucidate the relative contributions of AT1 versus AT2 cells to alveolar homeostasis. Cre has previously been efficiently expressed in AT2 cells in mouse lung with the surfactant protein (SP)-C promoter; however, no transgenic mouse expressing Cre in AT1 cells has so far been available. To develop an AT1 cell-specific transgenic Cre mouse, we generated a knockin of a Cre-IRES-DsRed cassette into exon 1 of the endogenous aquaporin 5 (Aqp5) gene, a gene expressed specifically in AT1 cells in the distal lung epithelium, resulting in the mouse line, Aqp5-Cre-IRES-DsRed (ACID). Endogenous Aqp5 and transgenic Cre in ACID mice showed a very similar pattern of tissue distribution by RT-PCR. To analyze Cre activity, ACID was crossed to two Cre reporter strains, R26LacZ and mT/mG. Double-transgenic offspring demonstrated reporter gene expression in a very high fraction of AT1 cells in the distal lung, whereas AT2 cells were negative. As expected, variable reporter expression was detected in several other tissues where endogenous Aqp5 is expressed (e.g., submandibular salivary gland and stomach). ACID mice should be of major utility in analyzing the functional contribution of AT1 cells to alveolar epithelial properties in vivo with Cre/loxP-mediated gene deletion technology.
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