ArticleGenome research2009
Relative contribution of sequence and structure features to the mRNA binding of Argonaute/EIF2C-miRNA complexes and the degradation of miRNA targets.
Article in Genome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 99 citations in OpenAlex.
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- Determinants of Functional MicroRNA Targeting.Molecules and cells · 2023Review
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- Emergence of Circulating MicroRNAs in Breast Cancer as Diagnostic and Therapeutic Efficacy Biomarkers.Molecular diagnosis & therapy · 2020Review
- Prediction of the miRNA interactome - Established methods and upcoming perspectives.Computational and structural biotechnology journal · 2020Review
- The biochemical basis of microRNA targeting efficacy.Science (New York, N.Y.) · 2019Article
- MiR-192, miR-200c and miR-17 are fibroblast-mediated inhibitors of colorectal cancer invasion.Oncotarget · 2018Article
- Predicting microRNA targeting efficacy in Drosophila.Genome biology · 2018Article
- Single-cell mRNA profiling reveals the hierarchical response of miRNA targets to miRNA induction.Molecular systems biology · 2018Article
- Translation and Translational Control in Dinoflagellates.Microorganisms · 2018Review
- Circulating microRNAs in breast cancer: novel diagnostic and prognostic biomarkers.Cell death & disease · 2017Review
- MicroRNA-495 Inhibits Gastric Cancer Cell Migration and Invasion Possibly via Targeting High Mobility Group AT-Hook 2 (HMGA2).Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Expression of microRNA‑26b and identification of its target gene EphA2 in pituitary tissues in Yanbian cattle.Molecular medicine reports · 2015Article
- Article
- Accurate transcriptome-wide prediction of microRNA targets and small interfering RNA off-targets with MIRZA-G.Nucleic acids research · 2015Article
- Identification and consequences of miRNA-target interactions--beyond repression of gene expression.Nature reviews. Genetics · 2014Review
- Systematic design and functional analysis of artificial microRNAs.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
How miRNAs recognize their target sites is a puzzle that many experimental and computational studies aimed to solve. Several features, such as perfect pairing of the miRNA seed, additional pairing in the 3' region of the miRNA, relative position in the 3' UTR, and the A/U content of the environment of the putative site, have been found to be relevant. Here we have used a large number of previously published data sets to assess the power that various sequence and structure features have in distinguishing between putative sites that do and those that do not appear to be functional. We found that although different data sets give widely different answers when it comes to ranking the relative importance of these features, the sites inferred from most transcriptomics experiments, as well as from comparative genomics, appear similar at this level. This suggests that miRNA target sites have been selected in evolution on their ability to trigger mRNA degradation. To understand at what step in the miRNA-induced response individual features play a role, we transfected human HEK293 cells with miRNAs and analyzed the association of Argonaute/EIF2C-miRNA complexes with target mRNAs and the degradation of these messages. We found that structural features of the target site are only important for Argonaute/EIF2C binding, while sequence features such as the A/U content of the 3' UTR are important for mRNA degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.