ArticlePlant physiology2009
Metabolite sorting of a germplasm collection reveals the hydroxylase3 locus as a new target for maize provitamin A biofortification.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 114 citations in OpenAlex.
- OsFT coordinates photosynthetic stability and carotenoid metabolism to regulate tillering in rice.Journal of advanced research · 2026Article
- Unraveling transcriptomics of sorghum grain carotenoids: a step forward for biofortification.BMC genomics · 2023Article
- Genetic and molecular basis of carotenoid metabolism in cereals.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Carotenoid modifying enzymes in metazoans.Methods in enzymology · 2022Article
- Differential hydroxylation efficiency of the two non-heme carotene hydroxylases: DcBCH1, rather than DcBCH2, plays a major role in carrot taproot.Horticulture research · 2022Article
- Enrichment of provitamin A content in durum wheat grain by suppressing β-carotene hydroxylase 1 genes with a TILLING approach.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Regulation of carotenoid and chlorophyll pools in hesperidia, anatomically unique fruits found only in Citrus.Plant physiology · 2021Article
- Development of Maize Hybrids With Enhanced Vitamin-E, Vitamin-A, Lysine, and Tryptophan Through Molecular Breeding.Frontiers in plant science · 2021Article
- Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity.Nature communications · 2020Article
- Structural basis for plant lutein biosynthesis from α-carotene.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Identification and epistasis analysis of quantitative trait loci for zeaxanthin concentration in maize kernel across different generations and environments.Breeding science · 2020Article
- Pro-vitamin A carotenoids in East African highland banana and otherFood science & nutrition · 2020Article
- A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat.The Plant cell · 2019Article
- Changing Form and Function through Carotenoids and Synthetic Biology.Plant physiology · 2019Article
- Development of Biofortified Maize Hybrids through Marker-Assisted Stacking of βFrontiers in plant science · 2018Article
- The Silencing of Carotenoid β-Hydroxylases by RNA Interference in Different Maize Genetic Backgrounds Increases the β-Carotene Content of the Endosperm.International journal of molecular sciences · 2017Article
- Vitamin B1 diversity and characterization of biosynthesis genes in cassava.Journal of experimental botany · 2017Article
- Article
- Identification and Expression Analysis of Candidate Genes Involved in Carotenoid Biosynthesis in Chickpea Seeds.Frontiers in plant science · 2016Article
- Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels.BMC plant biology · 2015Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Vitamin A deficiency, a global health burden, can be alleviated through provitamin A carotenoid biofortification of major crop staples such as maize (Zea mays) and other grasses in the Poaceae. If regulation of carotenoid biosynthesis was better understood, enhancement could be controlled by limiting beta-carotene hydroxylation to compounds with lower or no nonprovitamin A activity. Natural maize genetic diversity enabled identification of hydroxylation genes associated with reduced endosperm provitamin A content. A novel approach was used to capture the genetic and biochemical diversity of a large germplasm collection, representing 80% of maize genetic diversity, without having to sample the entire collection. Metabolite data sorting was applied to select a 10-line genetically diverse subset representing biochemical extremes for maize kernel carotenoids. Transcript profiling led to discovery of the Hydroxylase3 locus that coincidently mapped to a carotene quantitative trait locus, thereby prompting investigation of allelic variation in a broader collection. Three natural alleles in 51 maize lines explained 78% of variation and approximately 11-fold difference in beta-carotene relative to beta-cryptoxanthin and 36% of the variation and 4-fold difference in absolute levels of beta-carotene. A simple PCR assay to track and identify Hydroxylase3 alleles will be valuable for predicting nutritional content in genetically diverse cultivars found worldwide.
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