ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2010
Microfluidic, bead-based assay: Theory and experiments.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- An automated integrated platform for rapid and sensitive multiplexed protein profiling using human saliva samples.Lab on a chip · 2014Trial
- Numerical optimization of microfluidic biosensor detection time for the SARS-CoV-2 using the Taguchi method.Indian journal of physics and proceedings of the Indian Association for the Cultivation of Science (2004) · 2023Article
- Taguchi optimization of integrated flow microfluidic biosensor for COVID-19 detection.European physical journal plus · 2022Article
- Grasping and Releasing Agarose micro Beads in Water Drops.Micromachines · 2019Article
- Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions.Journal of visualized experiments : JoVE · 2018Article
- Transport of biomolecules to binding partners displayed on the surface of microbeads arrayed in traps in a microfluidic cell.Biomicrofluidics · 2017Article
- A bead-based fluorescence immunosensing technique enabled by the integration of Förster resonance energy transfer and optoelectrokinetic concentration.Biomicrofluidics · 2016Article
- Detection of autoantibodies using chemiluminescence technologies.Immunopharmacology and immunotoxicology · 2016Review
- Salivary diagnostics using a portable point-of-service platform: a review.Clinical therapeutics · 2015Review
- Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay.Arthritis research & therapy · 2013Article
- Effects of sample delivery on analyte capture in porous bead sensors.Lab on a chip · 2012Article
- Porous bead-based diagnostic platforms: bridging the gaps in healthcare.Sensors (Basel, Switzerland) · 2012Review
- Programmable bio-nano-chip systems for serum CA125 quantification: toward ovarian cancer diagnostics at the point-of-care.Cancer prevention research (Philadelphia, Pa.) · 2012Article
- Modeling analyte transport and capture in porous bead sensors.Analytical chemistry · 2012Article
- A Portable Analyzer for Pouch-Actuated, Immunoassay Cassettes.Sensors and actuators. B, Chemical · 2011Article
- Point-of-care oral-based diagnostics.Oral diseases · 2011Review
- A membrane-based, high-efficiency, microfluidic debubbler.Lab on a chip · 2011Article
- Pulsating bead-based assay.Analytical chemistry · 2011Article
- Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip.Small (Weinheim an der Bergstrasse, Germany) · 2011Article
- Polymeric microbead arrays for microfluidic applications.Journal of micromechanics and microengineering : structures, devices, and systems · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Microbeads are frequently used as a solid support for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. However, relatively few studies investigate the binding kinetics on modified bead surfaces in a microfluidics context. In this study, a customized hot embossing technique is used to stamp microwells in a thin plastic substrate where streptavidin-coated agarose beads are selectively placed and subsequently immobilized within a conduit. Biotinylated quantum dots are used as a label to monitor target analyte binding to the bead's surface. Three-dimensional finite element simulations are carried out to model the binding kinetics on the bead's surface. The model accounts for surface exclusion effects resulting from a single quantum dot occluding multiple receptor sites. The theoretical predictions are compared and favorably agree with experimental observations. The theoretical simulations provide a useful tool to predict how varying parameters affect microbead reaction kinetics and sensor performance. This study enhances our understanding of bead-based microfluidic assays and provides a design tool for developers of point-of-care, lab-on-chip devices for medical diagnosis, food and water quality inspection, and environmental monitoring.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.